ArticleThe Biochemical journal1999
Glucose and thyroid hormone co-regulate the expression of the intestinal fructose transporter GLUT5.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Hypothyroidism impairs the circadian rhythmicity of clock genes and proteins involved in gut nutrient absorption in female mice.Frontiers in physiology · 2025Article
- GLUT5: structure, functions, diseases and potential applications.Acta biochimica et biophysica Sinica · 2023Review
- Review
- Thyroid hormone signaling in the intestinal stem cells and their niche.Cellular and molecular life sciences : CMLS · 2022Review
- Article
- Regulation of the Fructose Transporter GeneInternational journal of molecular sciences · 2021Article
- Tissue-Specific Fructose Metabolism in Obesity and Diabetes.Current diabetes reports · 2020Review
- Glucose transporters in the small intestine in health and disease.Pflugers Archiv : European journal of physiology · 2020Review
- Identification of the fructose transporter GLUT5 (SLC2A5) as a novel target of nuclear receptor LXR.Scientific reports · 2019Article
- Glucose transporter expression in the human colon.World journal of gastroenterology · 2018Article
- The Role of Carbohydrate Response Element Binding Protein in Intestinal and Hepatic Fructose Metabolism.Nutrients · 2017Review
- ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance.Oncotarget · 2016Article
- Evidence of impaired carbohydrate assimilation in euthyroid patients with Hashimoto's thyroiditis.European journal of clinical nutrition · 2016Article
- Thyroid hormones and their nuclear receptors: new players in intestinal epithelium stem cell biology?Cellular and molecular life sciences : CMLS · 2014Review
- The role of thyroid hormone signaling in the prevention of digestive system cancers.International journal of molecular sciences · 2013Review
- MicroRNAs in pancreatic cancer metabolism.Nature reviews. Gastroenterology & hepatology · 2012Review
- Regulation of the fructose transporter GLUT5 in health and disease.American journal of physiology. Endocrinology and metabolism · 2008Review
- Thyroid hormone regulates the expression and function of P-glycoprotein in Caco-2 cells.Pharmaceutical research · 2008Article
- Thyroid hormone receptor alpha1 directly controls transcription of the beta-catenin gene in intestinal epithelial cells.Molecular and cellular biology · 2006Article
- Intestinal sugar transport.World journal of gastroenterology · 2006Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the fructose transporter GLUT5 in Caco-2 cells is controlled by the carbohydrate content of the culture media [Mesonero, Matosin, Cambier, Rodriguez-Yoldi and Brot-Laroche (1995) Biochem. J. 312, 757-762] and by the metabolic status of the cells [Mahraoui, Takeda, Mesonero, Chantret, Dussaulx, Bell, and Brot-Laroche (1994) Biochem. J. 301, 169-175]. In this study we show that, in fully differentiated Caco-2/TC7 cells, thyroid hormone and glucose increase GLUT5 mRNA abundance in a dose-dependent manner. Using Caco-2/TC7 cells stably transformed with various fragments of the GLUT5 promoter inserted upstream of the luciferase reporter gene, we localized the sequences that confer 3,3',5-l-tri-iodothyronine (T3)- and/or glucose-sensitivity to the gene. Glucose responsiveness is conferred by the -272/+41 fragment of the promoter, but it is only with the -338/+41 region that transcription of the luciferase reporter gene is stimulated by T3. This 70 bp fragment from position -338 to -272 of the GLUT5 gene is able to confer T3/glucose-responsiveness to the heterologous thymidine kinase promoter. Electrophoretic-mobility-shift assays demonstrate that thyroid hormone receptors alpha and beta are expressed in Caco-2/TC7 cells. They further show that the -308/-290 region of the GLUT5 promoter binds thyroid hormone receptor/retinoid X receptor heterodimers, and that glucose and/or T3 exert a deleterious effect on the binding of the nuclear protein complex.
Indexed as
Identifiers
10191252PMC1220150W2094563019What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.