ArticleThe Journal of cell biology1999
Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMP-II homologue.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 47 citations in OpenAlex.
- The Amoebal Model for Macropinocytosis.Sub-cellular biochemistry · 2022Article
- CaCells · 2020Review
- A tripeptidyl peptidase 1 is a binding partner of the Golgi pH regulator (GPHR) inDisease models & mechanisms · 2017Article
- Protein mediators of sterol transport across intestinal brush border membrane.Sub-cellular biochemistry · 2010Review
- High-resolution dissection of phagosome maturation reveals distinct membrane trafficking phases.Molecular biology of the cell · 2002Article
- Dictyostelium LvsB mutants model the lysosomal defects associated with Chediak-Higashi syndrome.Molecular biology of the cell · 2002Article
- Sequential activities of phosphoinositide 3-kinase, PKB/Aakt, and Rab7 during macropinosome formation in Dictyostelium.Molecular biology of the cell · 2001Article
- Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development.The Plant cell · 2001Article
- Inactivation of lmpA, encoding a LIMPII-related endosomal protein, suppresses the internalization and endosomal trafficking defects in profilin-null mutants.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Profilin is an ubiquitous G-actin binding protein in eukaryotic cells. Lack of both profilin isoforms in Dictyostelium discoideum resulted in impaired cytokinesis and an arrest in development. A restriction enzyme-mediated integration approach was applied to profilin-minus cells to identify suppressor mutants for the developmental phenotype. A mutant with wild-type-like development and restored cytokinesis was isolated. The gene affected was found to code for an integral membrane glycoprotein of a predicted size of 88 kD containing two transmembrane domains, one at the NH2 terminus and the other at the COOH terminus. It is homologous to mammalian CD36/LIMP-II and represents the first member of this family in D. discoideum, therefore the name DdLIMP is proposed. Targeted disruption of the lmpA gene in the profilin-minus background also rescued the mutant phenotype. Immunofluorescence revealed a localization in vesicles and ringlike structures on the cell surface. Partially purified DdLIMP bound specifically to PIP2 in sedimentation and gel filtration assays. A direct interaction between DdLIMP and profilin could not be detected, and it is unclear how far upstream in a regulatory cascade DdLIMP might be positioned. However, the PIP2 binding of DdLIMP points towards a function via the phosphatidylinositol pathway, a major regulator of profilin.
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