ArticleBritish journal of pharmacology1999
Modulation of ATP-responses at recombinant rP2X4 receptors by extracellular pH and zinc.
Article in British journal of pharmacology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- The P2X4 Receptor: Cellular and Molecular Characteristics of a Promising Neuroinflammatory Target.International journal of molecular sciences · 2022Review
- The Function and Regulation of Zinc in the Brain.Neuroscience · 2021Review
- New Insights of the Zn(II)-Induced P2 × 4R Positive Allosteric Modulation: Role of Head Receptor Domain SS2/SS3, E160 and D170.International journal of molecular sciences · 2020Article
- The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy.Frontiers in pharmacology · 2019Article
- P2XThe Journal of physiology · 2018Article
- P2X4 Receptor Function in the Nervous System and Current Breakthroughs in Pharmacology.Frontiers in pharmacology · 2017Review
- Zinc as Allosteric Ion Channel Modulator: Ionotropic Receptors as Metalloproteins.International journal of molecular sciences · 2016Review
- Heteromeric assembly of P2X subunits.Frontiers in cellular neuroscience · 2013Review
- Tryptophan 46 is a site for ethanol and ivermectin action in P2X4 receptors.Purinergic signalling · 2013Article
- Positive allosteric modulation by ivermectin of human but not murine P2X7 receptors.British journal of pharmacology · 2012Article
- A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening.Structure (London, England : 1993) · 2012Article
- ATP-activated P2X2 current in mouse spermatozoa.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Receptor-like activity evoked by extracellular ADP in Arabidopsis root epidermal plasma membrane.Plant physiology · 2011Article
- Functional expression of P2X4 receptor in capillary endothelial cells of the cochlear spiral ligament and its role in regulating the capillary diameter.American journal of physiology. Heart and circulatory physiology · 2011Article
- P2X(7) receptor at the heart of disease.Hippokratia · 2010Article
- ENaC, renal sodium excretion and extracellular ATP.Purinergic signalling · 2009Article
- Inhibition of P2X(7) receptors by divalent cations: old action and new insight.European biophysics journal : EBJ · 2009Review
- Identification of the amino acid residues in the extracellular domain of rat P2X(7) receptor involved in functional inhibition by acidic pH.British journal of pharmacology · 2009Article
- Contribution of extracellular negatively charged residues to ATP action and zinc modulation of rat P2X2 receptors.Journal of neurochemistry · 2008Article
- Trace metals in the brain: allosteric modulators of ligand-gated receptor channels, the case of ATP-gated P2X receptors.European biophysics journal : EBJ · 2008Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The modulatory effects of extracellular H+ and Zn2+ were tested against ATP-responses at rat P2X4 (rP2X4) receptors expressed in Xenopus oocytes under voltage-clamp conditions. ATP (0.1-100 microM, at pH 7.5), evoked inward currents via rP2X4 receptors (EC50 value, 4.1+/-0.98 microM; nH, 1.2+/-0.1). ATP potency was reduced 2 fold, at pH 6.5, without altering maximal activity. ATP potency was reduced by a further 4 fold, at pH 5.5, and the maximal activity of ATP was also reduced. Alkaline conditions (pH 8.0) had no effect on ATP-responses. Zn2+ (100 nM - 10 microM) potentiated ATP-responses at the rP2X4 receptor by 2 fold, whereas higher concentrations (30 microM - 1 mM) inhibited ATP-responses. Zn2+ potentiation was due to an increase in ATP potency, whereas its inhibitory action was due to a reduction in ATP efficacy. Zn2+ modulation of ATP-responses was pH-dependent. At pH 6.5, the bell-shaped curve for Zn2+ was shifted to the right by 1 log unit. At pH 5.5, Zn2+ potentiation was abolished and its inhibitory effect reduced considerably. Suramin (50 microM) also potentiated ATP-responses at rP2X4 receptors. Neither H+ (pH 6.5 and 5.5), Zn2+ (10-100 microM) or a combination of both failed to reveal an inhibitory action of suramin at rP2X4 receptors. In conclusion, H+ and Zn2+ exerted opposite effects on the rP2X4 receptor by lowering and raising agonist potency, respectively. H+ (> or = 3 microM) and Zn2+ (> or = 30 microM) also reduces agonist efficacy by lowering the number of rP2X4 receptors available for activation. The striking differences between the modulatory actions of H+ and Zn2+ at rP2X4 and rP2X2 receptors are discussed.
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