ArticleApplied and environmental microbiology1999
Development and characterization of a fluorescent-bacteriophage assay for detection of Escherichia coli O157:H7.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Engineered bacteriophage-based bioimaging Technology: Development and applications.Synthetic and systems biotechnology · 2025Review
- Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics.Future microbiology · 2024Review
- Recombinant Reporter Phage rTUN1::ACS sensors · 2023Article
- Water-in-oil droplet-mediated method for detecting and isolating infectious bacteriophage particles via fluorescent staining.Frontiers in microbiology · 2023Article
- Advance on Engineering of Bacteriophages by Synthetic Biology.Infection and drug resistance · 2023Review
- Review
- Bacteriophage Based Biosensors: Trends, Outcomes and Challenges.Nanomaterials (Basel, Switzerland) · 2020Review
- Nano-etched fused-silica capillary used for on-line preconcentration and electrophoretic separation of bacteriophages from large blood sample volumes with off-line MALDI-TOF mass spectrometry identification.Mikrochimica acta · 2020Article
- Fast and Easy Phage-Tagging and Live/Dead Analysis for the Rapid Monitoring of Bacteriophage Infection.Frontiers in microbiology · 2020Article
- Colorimetric detection of Escherichia coli using engineered bacteriophage and an affinity reporter system.Analytical and bioanalytical chemistry · 2019Article
- Development of a bacteriophage-based Method for Detection ofFood safety (Tokyo, Japan) · 2018Article
- Detection Methodologies for Pathogen and Toxins: A Review.Sensors (Basel, Switzerland) · 2017Review
- Engineering bacteriophage for a pragmatic low-resource setting bacterial diagnostic platform.Bioengineered · 2016Article
- Detection of viruses by counting single fluorescent genetically biotinylated reporter immunophage using a lateral flow assay.ACS applied materials & interfaces · 2015Article
- Article
- Application of bacteriophages for detection of foodborne pathogens.Bacteriophage · 2014Review
- The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5.Virology journal · 2013Article
- Recent advances in bacteriophage based biosensors for food-borne pathogen detection.Sensors (Basel, Switzerland) · 2013Review
- Article
- Phage-based platforms for the clinical detection of human bacterial pathogens.Bacteriophage · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper we describe evaluation and characterization of a novel assay that combines immunomagnetic separation and a fluorescently stained bacteriophage for detection of Escherichia coli O157:H7 in broth. When it was combined with flow cytometry, the fluorescent-bacteriophage assay (FBA) was capable of detecting 10(4) cells/ml. A modified direct epifluorescent-filter technique (DEFT) was employed in an attempt to estimate bacterial concentrations. Using regression analysis, we calculated that the lower detection limit was between 10(2) and 10(3) cells/ml; however, the modified DEFT was found to be an unreliable method for determining bacterial concentrations. The results of this study show that the FBA, when combined with flow cytometry, is a sensitive technique for presumptive detection of E. coli O157:H7 in broth cultures.
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