ArticleGenetics1999
Specific components of the SAGA complex are required for Gcn4- and Gcr1-mediated activation of the his4-912delta promoter in Saccharomyces cerevisiae.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Differential activation mechanisms of two isoforms of Gcr1 transcription factor generated from spliced and un-spliced transcripts in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Truncated, strong inducible promoter P3 Biotech · 2019Article
- An Autophagy-Independent Role forGenetics · 2018Article
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- The Histone Acetyltransferase Gcn5 Regulates ncRNA-ICR1 and FLO11 Expression during Pseudohyphal Development in Saccharomyces cerevisiae.BioMed research international · 2015Article
- Site-specific cross-linking of TBP in vivo and in vitro reveals a direct functional interaction with the SAGA subunit Spt3.Genes & development · 2008Article
- Remodeling yeast gene transcription by activating the Ty1 long terminal repeat retrotransposon under severe adenine deficiency.Molecular and cellular biology · 2008Article
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- Regulation of an intergenic transcript controls adjacent gene transcription in Saccharomyces cerevisiae.Genes & development · 2005Article
- Severe adenine starvation activates Ty1 transcription and retrotransposition in Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- Global position and recruitment of HATs and HDACs in the yeast genome.Molecular cell · 2004Article
- Enhancer choice in cis and in trans in Drosophila melanogaster: role of the promoter.Genetics · 2004Article
- Molecular requirements for gene expression mediated by targeted histone acetyltransferases.Molecular and cellular biology · 2004Article
- Local definition of Ty1 target preference by long terminal repeats and clustered tRNA genes.Genome research · 2004Article
- Identification, mutational analysis, and coactivator requirements of two distinct transcriptional activation domains of the Saccharomyces cerevisiae Hap4 protein.Eukaryotic cell · 2004Article
- Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatin.Molecular and cellular biology · 2004Article
- Two Drosophila Ada2 homologues function in different multiprotein complexes.Molecular and cellular biology · 2003Article
- Mutations in GCR1 affect SUC2 gene expression in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2003Article
- A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B.The EMBO journal · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mutations selected as suppressors of Ty or solo delta insertion mutations in Saccharomyces cerevisiae have identified several genes, SPT3, SPT7, SPT8, and SPT20, that encode components of the SAGA complex. However, the mechanism by which SAGA activates transcription of specific RNA polymerase II-dependent genes is unknown. We have conducted a fine-structure mutagenesis of one widely used SAGA-dependent promoter, the delta element of his4-912delta, to identify sequence elements important for its promoter activity. Our analysis has characterized three delta regions necessary for full promoter activity and accurate start site selection: an upstream activating sequence, a TATA region, and an initiator region. In addition, we have shown that factors present at the adjacent UASHIS4 (Gcn4, Bas1, and Pho2) also activate the delta promoter in his4-912delta. Our results suggest a model in which the delta promoter in his4-912delta is primarily activated by two factors: Gcr1 acting at the UASdelta and Gcn4 acting at the UASHIS4. Finally, we tested whether activation by either of these factors is dependent on components of the SAGA complex. Our results demonstrate that Spt3 and Spt20 are required for full delta promoter activity, but that Gcn5, another member of SAGA, is not required. Spt3 appears to be partially required for activation of his4-912delta by both Gcr1 and Gcn4. Thus, our work suggests that SAGA exerts a large effect on delta promoter activity through a combination of smaller effects on multiple factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.