ArticleGenetics1999
Analysis of mutations in the yeast mRNA decapping enzyme.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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Who cites it
45 citing papers in PubMed, 75 citations in OpenAlex.
- The RGG motif proteins: Interactions, functions, and regulations.Wiley interdisciplinary reviews. RNA · 2023Review
- General decapping activators target different subsets of inefficiently translated mRNAs.eLife · 2018Article
- Structural and molecular mechanisms for the control of eukaryotic 5'-3' mRNA decay.Nature structural & molecular biology · 2018Review
- The S. pombe mRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface.RNA (New York, N.Y.) · 2016Article
- Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation.Nature structural & molecular biology · 2016Article
- Not4-dependent translational repression is important for cellular protein homeostasis in yeast.The EMBO journal · 2015Article
- The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1.Nucleic acids research · 2014Article
- RNA degradation in Saccharomyces cerevisae.Genetics · 2012Review
- Dehydration stress activates Arabidopsis MPK6 to signal DCP1 phosphorylation.The EMBO journal · 2012Article
- SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA deadenylation.American journal of molecular biology · 2012Article
- Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition.RNA (New York, N.Y.) · 2011Article
- 5' to 3' mRNA decay factors colocalize with Ty1 gag and human APOBEC3G and promote Ty1 retrotransposition.Journal of virology · 2010Article
- Activation of decapping involves binding of the mRNA and facilitation of the post-binding steps by the Lsm1-7-Pat1 complex.RNA (New York, N.Y.) · 2009Article
- The control of mRNA decapping and P-body formation.Molecular cell · 2008Review
- A novel class of mRNA-containing cytoplasmic granules are produced in response to UV-irradiation.Molecular biology of the cell · 2008Article
- lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo.RNA (New York, N.Y.) · 2008Article
- Structural basis of dcp2 recognition and activation by dcp1.Molecular cell · 2008Article
- Analysis of cytoplasmic mRNA decay in Saccharomyces cerevisiae.Methods in enzymology · 2008Article
- The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs.RNA (New York, N.Y.) · 2007Article
- Arabidopsis DCP2, DCP1, and VARICOSE form a decapping complex required for postembryonic development.The Plant cell · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major mechanism of mRNA decay in yeast is initiated by deadenylation, followed by mRNA decapping, which exposes the transcript to 5' to 3' exonucleolytic degradation. The decapping enzyme that removes the 5' cap structure is encoded by the DCP1 gene. To understand the function of the decapping enzyme, we used alanine scanning mutagenesis to create 31 mutant versions of the enzyme, and we examined the effects of the mutations both in vivo and in vitro. Two types of mutations that affected mRNA decapping in vivo were identified, including a temperature-sensitive allele. First, two mutants produced decapping enzymes that were defective for decapping in vitro, suggesting that these mutated residues are required for enzymatic activity. In contrast, several mutants that moderately affected mRNA decapping in vivo yielded decapping enzymes that had at least the same specific activity as the wild-type enzyme in vitro. Combination of alleles within this group yielded decapping enzymes that showed a strong loss of function in vivo, but that still produced fully active enzymes in vitro. This suggested that interactions of the decapping enzyme with other factors may be required for efficient decapping in vivo, and that these particular mutations may be disrupting such interactions. Interestingly, partial loss of decapping activity in vivo led to a defect in normal deadenylation-dependent decapping, but it did not affect the rapid deadenylation-independent decapping triggered by early nonsense codons. This observation suggested that these two types of mRNA decapping differ in their requirements for the decapping enzyme.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.