ArticleGenetics1999
CYS3, a hotspot of meiotic recombination in Saccharomyces cerevisiae. Effects of heterozygosity and mismatch repair functions on gene conversion and recombination intermediates.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Programming sites of meiotic crossovers using Spo11 fusion proteins.Nucleic acids research · 2017Article
- Locally, meiotic double-strand breaks targeted by Gal4BD-Spo11 occur at discrete sites with a sequence preference.Molecular and cellular biology · 2009Article
- Gel electrophoresis assays for analyzing DNA double-strand breaks in Saccharomyces cerevisiae at various spatial resolutions.Methods in molecular biology (Clifton, N.J.) · 2009Review
- Pds1p is required for meiotic recombination and prophase I progression in Saccharomyces cerevisiae.Genetics · 2009Article
- Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination.Genes & development · 2008Article
- Meiotic crossover hotspots contained in haplotype block boundaries of the mouse genome.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114.Nucleic acids research · 2007Article
- Article
- Evolutionary mechanisms shaping the genomic structure of the Williams-Beuren syndrome chromosomal region at human 7q11.23.Genome research · 2005Article
- Systematic mutagenesis of the Saccharomyces cerevisiae MLH1 gene reveals distinct roles for Mlh1p in meiotic crossing over and in vegetative and meiotic mismatch repair.Molecular and cellular biology · 2003Article
- Replication protein A is required for meiotic recombination in Saccharomyces cerevisiae.Genetics · 2002Article
- Article
- Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined meiotic recombination at the CYS3 locus. Genetic analysis indicates that CYS3 is a hotspot of meiotic gene conversion, with a putative 5'-3' polarity gradient of conversion frequencies. This gradient is relieved in the presence of msh2 and pms1 mutations, indicating an involvement of mismatch repair functions in meiotic recombination. To investigate the role of mismatch repair proteins in meiotic recombination, we performed a physical analysis of meiotic DNA in wild-type and msh2 pms1 strains in the presence or absence of allelic differences at CYS3. Neither the mutations in CYS3 nor the absence of mismatch repair functions affects the frequency and distribution of nearby recombination-initiating DNA double-strand breaks (DSBs). Processing of DSBs is also similar in msh2 pms1 and wild-type strains. We conclude that mismatch repair functions do not control the distribution of meiotic gene conversion events at the initiating steps. In the MSH2 PMS1 background, strains heteroallelic for frameshift mutations in CYS3 exhibit a frequency of gene conversion greater than that observed for either marker alone. Physical analysis revealed no modification in the formation of DSBs, suggesting that this marker effect results from subsequent processing events that are not yet understood.
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