ArticleEuropean journal of cell biology1999
2E4 (kaptin): a novel actin-associated protein from human blood platelets found in lamellipodia and the tips of the stereocilia of the inner ear.
Article in European journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Nonsense variant in a consanguineous family expands the phenotype of KPTN gene-related syndrome to include hearing impairment.Clinical genetics · 2023Article
- Mutations in KPTN cause macrocephaly, neurodevelopmental delay, and seizures.American journal of human genetics · 2014Article
- There is more than one way to model an elephant. Experiment-driven modeling of the actin cytoskeleton.Biophysical journal · 2013Review
- New structural and functional contexts of the Dx[DN]xDG linear motif: insights into evolution of calcium-binding proteins.PloS one · 2011Article
- Imipramine treatment and resiliency exhibit similar chromatin regulation in the mouse nucleus accumbens in depression models.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- beta-Actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Article
- Actin dynamics in platelets.International review of cytology · 2002Review
- Motility-related proteins as markers for head and neck squamous cell cancer.The Laryngoscope · 2001Article
- VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations.Cell motility and the cytoskeleton · 2000Article
- 2E4/Kaptin (KPTN)--a candidate gene for the hearing loss locus, DFNA4.Annals of human genetics · 2000Article
- Parallel actin bundles and their multiple actin-bundling proteins.Current opinion in cell biology · 2000Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Platelet activation, crucial for hemostasis, requires actin polymerization, yet the molecular mechanisms by which localized actin polymerization is mediated are not clear. Here we report the characterization of a novel actin-binding protein, 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. 2E4 binds to filamentous (F)-actin by F-actin affinity chromatography and is eluted from F-actin affinity columns and extracted from cells with ATP. Its presence at the leading edge of platelets spread on glass and in the lamellipodia of motile fibroblasts suggests a role in actin dynamics. Using localization to obtain clues about function, we stained the sensory epithelium of the embryonic inner ear to determine whether 2E4 is at the barbed end of actin filaments during their elongation. Indeed, 2E4 was present at the tips of the elongating stereocilium. 2E4 is novel by DNA sequence and has no identifiable structural motifs. Its unusual amino acid sequence, its ATP-sensitive actin association and its location at sites of actin polymerization in cells suggest 2E4 plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation.
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