ArticleMolecular and cellular biochemistry1999
Detection of a sequence involved in actin-binding and phosphoinositide-binding in the N-terminal side of cofilin.
Article in Molecular and cellular biochemistry, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- The potassium channel KNature communications · 2025Article
- Lipid Polarization during Cytokinesis.Cells · 2022Review
- Membrane and organelle dynamics during cell division.Nature reviews. Molecular cell biology · 2020Review
- Cofilin, a Master Node Regulating Cytoskeletal Pathogenesis in Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2019Review
- Structural basis for cofilin binding and actin filament disassembly.Nature communications · 2018Article
- Phosphoinositide Binding Inhibits Actin Crosslinking and Polymerization by Palladin.Journal of molecular biology · 2016Article
- Pharmacologic regulation of AMPK in breast cancer affects cytoskeletal properties involved with microtentacle formation and re-attachment.Oncotarget · 2015Article
- ADP-ribosylation factor 6 (ARF6) bidirectionally regulates dendritic spine formation depending on neuronal maturation and activity.The Journal of biological chemistry · 2015Article
- Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction.The Journal of biological chemistry · 2013Article
- Article
- Role of phosphoinositides at the neuronal synapse.Sub-cellular biochemistry · 2012Review
- Solution structure and dynamics of ADF from Toxoplasma gondii.Journal of structural biology · 2011Article
- Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction.Molecular & cellular proteomics : MCP · 2011Article
- ADF/cofilin binds phosphoinositides in a multivalent manner to act as a PIP(2)-density sensor.Biophysical journal · 2010Article
- Phosphatidylinositol (4,5)-bisphosphate regulation of N-methyl-D-aspartate receptor channels in cortical neurons.Molecular pharmacology · 2009Article
- Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines.The open neuroscience journal · 2009Article
- Activity of cofilin can be regulated by a mechanism other than phosphorylation/dephosphorylation in muscle cells in culture.Journal of muscle research and cell motility · 2007Article
- Actin-depolymerizing factor mediates Rac/Rop GTPase-regulated pollen tube growth.The Plant cell · 2003Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cofilin is an actin-binding protein of low molecular weight which is widely distributed in eukaryotes and is deeply involved in the dynamics of actin assembly in the cytoplasm. The actin-binding ability of cofilin is inhibited by inositol phosphates (PIP2), and the PIP2- and actin-binding site(s) has been localized in residues W104-M115 of the cofilin primary sequence (Yonezawa et al. 1991 ). In the present study, in order to further clarify the functional domains in cofilin molecule, we constructed expression vectors containing cDNAs of different size with deletion at the 3'-region of the open reading frame. The truncated cofilin molecules produced in E. coli were purified and examined for their actin-binding and PIP2-binding ability. We found that the truncated cofilin molecule without C-terminal residues #100-#166 including the previously-described actin-binding site could be cross-linked with actin by EDC, a zero-length cross-linker. In addition, these truncated peptides as well as synthetic peptides corresponding to the N-terminal sequence of cofilin suppressed the inhibitory action of PIP2 on actin-cofilin interaction. These results strongly suggest that additional actin- and PIP2-binding sites exist in the N-terminal region of cofilin.
Indexed as
Identifiers
10098980W201083820What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.