ArticleProceedings of the National Academy of Sciences of the United States of America1999
Identification of the block in targeted retroviral-mediated gene transfer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- Optimizing In Vivo CAR T-cell Engineering for Cancer Immunotherapy.Cancer research · 2026Review
- Roles of phosphatidylserine exposed on the viral envelope and cell membrane in HIV-1 replication.Cell communication and signaling : CCS · 2019Review
- Versatile targeting system for lentiviral vectors involving biotinylated targeting molecules.Virology · 2018Article
- Library screening and receptor-directed targeting of gammaretroviral vectors.Future microbiology · 2013Review
- Article
- Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins.Journal of virology · 2012Article
- Receptors and tropisms of envelope viruses.Current opinion in virology · 2011Review
- The soluble serum protein Gas6 bridges virion envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry.Cell host & microbe · 2011Article
- Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from Autographa californica GP64 and Sendai virus F2 domain.BMC biotechnology · 2009Article
- Targeted gene delivery to CD117-expressing cells in vivo with lentiviral vectors co-displaying stem cell factor and a fusogenic molecule.Biotechnology and bioengineering · 2009Article
- Single-round selection yields a unique retroviral envelope utilizing GPR172A as its host receptor.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The effects of N-terminal insertion into VSV-G of an scFv peptide.Virology journal · 2006Article
- Rapid adaptation of a recombinant vesicular stomatitis virus to a targeted cell line.Journal of virology · 2006Article
- Multiple modifications allow high-titer production of retroviral vectors carrying heterologous regulatory elements.Journal of virology · 2004Article
- Factors affecting the direct targeting of murine leukemia virus vectors containing peptide ligands in the envelope protein.EMBO reports · 2002Article
- Selective targeting and inducible destruction of human cancer cells by retroviruses with envelope proteins bearing short peptide ligands.Journal of virology · 2002Article
- Redirecting retroviral tropism by insertion of short, nondisruptive peptide ligands into envelope.Journal of virology · 2002Article
- Two point mutations produce infectious retrovirus bearing a green fluorescent protein-SU fusion protein.Journal of virology · 2001Article
- Receptor binding transforms the surface subunit of the mammalian C-type retrovirus envelope protein from an inhibitor to an activator of fusion.Journal of virology · 2001Article
- Antibody-directed targeting of retroviral vectors via cell surface antigens.Journal of virology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
A chimeric retroviral vector (33E67) containing a CD33-specific single-chain antibody was generated in an attempt to target cells displaying the CD33 surface antigen. The chimeric envelope protein was translated, processed, and incorporated into viral particles as efficiently as wild-type envelope protein. The viral particles carrying the 33E67 envelope protein could bind efficiently to the CD33 receptor on target cells and were internalized, but no gene transfer occurred. A unique experimental approach was used to examine the basis for this postbinding block. Our data indicate that the chimeric envelope protein itself cannot participate in the fusion process, the most reasonable explanation being that this chimeric protein cannot undergo the appropriate conformational change that is thought to be triggered by receptor binding, a suggested prerequisite to subsequent fusion and core entry. These results indicate that the block to gene transfer in this system, and probably in most of the current chimeric retroviral vectors to date, is the inability of the chimeric envelope protein to undergo this obligatory conformational change.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.