ArticleProceedings of the National Academy of Sciences of the United States of America1999
An exon that prevents transport of a mature mRNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Regulation of mRNA stability contributes to the function of innate lymphoid cells in various diseases.Frontiers in immunology · 2023Review
- Evolutionarily conserved autoregulation of alternative pre-mRNA splicing by ribosomal protein L10a.Nucleic acids research · 2016Article
- Identification and characterization of alternative splicing in parasitic nematode transcriptomes.Parasites & vectors · 2014Article
- Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans.Nature protocols · 2010Article
- U2AF binding selects for the high conservation of the C. elegans 3' splice site.RNA (New York, N.Y.) · 2005Article
- UAP56 levels affect viability and mRNA export in Caenorhabditis elegans.RNA (New York, N.Y.) · 2003Article
- The human papillomavirus type 31 late 3' untranslated region contains a complex bipartite negative regulatory element.Journal of virology · 2002Article
- Regulation of nuclear gamma interferon gene expression by interleukin 12 (IL-12) and IL-2 represents a novel form of posttranscriptional control.Molecular and cellular biology · 2002Article
- Characterization of U2AF(6), a splicing factor related to U2AF(35).Molecular and cellular biology · 2002Article
- Functional selection of splicing enhancers that stimulate trans-splicing in vitro.RNA (New York, N.Y.) · 2001Article
- 3' splice site recognition in nematode trans-splicing involves enhancer-dependent recruitment of U2 snRNP.RNA (New York, N.Y.) · 2001Article
- Caspase-2 pre-mRNA alternative splicing: Identification of an intronic element containing a decoy 3' acceptor site.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Nuclear export of heat shock and non-heat-shock mRNA occurs via similar pathways.Molecular and cellular biology · 2000Article
- The human papillomavirus type 16 negative regulatory RNA element interacts with three proteins that act at different posttranscriptional levels.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
In Caenorhabditis elegans, pre-mRNA for the essential splicing factor U2AF65 sometimes is spliced to produce an RNA that includes an extra 216-bp internal exon, exon 3. Inclusion of exon 3 inserts an in-frame stop codon, yet this RNA is not subject to SMG-mediated RNA surveillance. To test whether exon 3 causes RNA to remain nuclear and thereby escape decay, we inserted it into the 3' untranslated region of a gfp reporter gene. Although exon 3 did not affect accumulation or processing of the mRNA, it dramatically suppressed expression of green fluorescent protein (GFP). We showed by in situ hybridization that exon 3-containing gfp RNA is retained in the nucleus. Intriguingly, exon 3 contains 10 matches to the 8-bp 3' splice-site consensus. We hypothesized that U2AF might recognize this octamer and thereby prevent export. This idea is supported by RNA interference experiments in which reduced levels of U2AF resulted in a small burst of gfp expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.