ArticleProceedings of the National Academy of Sciences of the United States of America1999
Genomic evolution during a 10,000-generation experiment with bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed.
- The complex genomic diversity ofEcology and evolution · 2023Article
- Revisiting the Design of the Long-Term Evolution Experiment with Escherichia coli.Journal of molecular evolution · 2023Review
- Attempts to limit sporulation in the probiotic strainAccess microbiology · 2023Article
- Discovery of positive and purifying selection in metagenomic time series of hypermutator microbial populations.PLoS genetics · 2022Article
- The dynamic network of IS30 transposition pathways.PloS one · 2022Article
- Adaptation and genomic erosion in fragmented Pseudomonas aeruginosa populations in the sinuses of people with cystic fibrosis.Cell reports · 2021Article
- Selection Maintains Protein Interactome Resilience in the Long-Term Evolution Experiment with Escherichia coli.Genome biology and evolution · 2021Article
- Long-Term m5C Methylome Dynamics Parallel Phenotypic Adaptation in the Cyanobacterium Trichodesmium.Molecular biology and evolution · 2021Article
- Insertion-sequence-mediated mutations both promote and constrain evolvability during a long-term experiment with bacteria.Nature communications · 2021Article
- Molecular Characterization ofFoods (Basel, Switzerland) · 2020Article
- How industrial bacterial cultures can be kept stable over time.Letters in applied microbiology · 2020Review
- Divergent Evolution of Mutation Rates and Biases in the Long-Term Evolution Experiment with Escherichia coli.Genome biology and evolution · 2020Article
- Genomic and phenotypic evolution ofeLife · 2020Article
- Evolutionary Rescue of an EnvironmentalFrontiers in microbiology · 2020Article
- Trusting the hand that feeds: microbes evolve to anticipate a serial transfer protocol as individuals or collectives.BMC evolutionary biology · 2019Article
- Distribution of fitness effects of mutations obtained from a simple genetic regulatory network model.Scientific reports · 2019Article
- Article
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- Investigating the genetic and epigenetic basis of big biological questions with the parthenogenetic marbled crayfish: A review and perspectives.Journal of biosciences · 2018Review
- Insertion element mediatedInfection and drug resistance · 2018Article
39 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular methods are used widely to measure genetic diversity within populations and determine relationships among species. However, it is difficult to observe genomic evolution in action because these dynamics are too slow in most organisms. To overcome this limitation, we sampled genomes from populations of Escherichia coli evolving in the laboratory for 10,000 generations. We analyzed the genomes for restriction fragment length polymorphisms (RFLP) using seven insertion sequences (IS) as probes; most polymorphisms detected by this approach reflect rearrangements (including transpositions) rather than point mutations. The evolving genomes became increasingly different from their ancestor over time. Moreover, tremendous diversity accumulated within each population, such that almost every individual had a different genetic fingerprint after 10,000 generations. As has been often suggested, but not previously shown by experiment, the rates of phenotypic and genomic change were discordant, both across replicate populations and over time within a population. Certain pivotal mutations were shared by all descendants in a population, and these are candidates for beneficial mutations, which are rare and difficult to find. More generally, these data show that the genome is highly dynamic even over a time scale that is, from an evolutionary perspective, very brief.
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