ArticleProceedings of the National Academy of Sciences of the United States of America1999
Morphogenetic movements at gastrulation require the SH2 tyrosine phosphatase Shp2.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 89 citations in OpenAlex.
- From Stem to Sternum: The Role of Shp2 in the Skeleton.Calcified tissue international · 2023Review
- Innovative therapeutic strategies for cardiovascular disease.EXCLI journal · 2023Review
- Article
- SHP2-Mediated Signal Networks in Stem Cell Homeostasis and Dysfunction.Stem cells international · 2018Review
- Spina Bifida: Pathogenesis, Mechanisms, and Genes in Mice and Humans.Scientifica · 2017Review
- Novel Mode of Defective Neural Tube Closure in the Non-Obese Diabetic (NOD) Mouse Strain.Scientific reports · 2015Article
- The Q510E mutation in Shp2 perturbs heart valve development by increasing cell migration.Journal of applied physiology (Bethesda, Md. : 1985) · 2015Article
- PZR coordinates Shp2 Noonan and LEOPARD syndrome signaling in zebrafish and mice.Molecular and cellular biology · 2014Article
- Traffic jam in the primitive streak: the role of defective mesoderm migration in birth defects.Birth defects research. Part A, Clinical and molecular teratology · 2014Review
- SHP-2 deletion in postmigratory neural crest cells results in impaired cardiac sympathetic innervation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Phosphoproteomics-mediated identification of Fer kinase as a target of mutant Shp2 in Noonan and LEOPARD syndrome.PloS one · 2014Article
- Review
- Regulation of endothelial cell differentiation and specification.Circulation research · 2013Review
- Shp2 plays a crucial role in cell structural orientation and force polarity in response to matrix rigidity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- SHP-2 acts via ROCK to regulate the cardiac actin cytoskeleton.Development (Cambridge, England) · 2012Article
- Xenopus: An emerging model for studying congenital heart disease.Birth defects research. Part A, Clinical and molecular teratology · 2011Review
- DNA methyltransferase inhibition induces mouse embryonic stem cell differentiation into endothelial cells.Experimental cell research · 2010Article
- Shp2 acts downstream of SDF-1alpha/CXCR4 in guiding granule cell migration during cerebellar development.Developmental biology · 2009Article
- Protein tyrosine phosphatase SHP-2: a proto-oncogene product that promotes Ras activation.Cancer science · 2009Review
- Embryonic vasculogenesis and hematopoietic specification.Organogenesis · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SH2 domain-containing tyrosine phosphatase Shp2 plays a pivotal role during the gastrulation of vertebrate embryos. However, because of the complex phenotype observed in mouse mutant embryos, the precise role of Shp2 during development is unclear. To define the specific functions of this phosphatase, Shp2 homozygous mutant embryonic stem cells bearing the Rosa-26 LacZ transgene were isolated and used to perform a chimeric analysis. Here, we show that Shp2 mutant cells amass in the tail bud of embryonic day 10.5 chimeric mouse embryos and that this accumulation begins at the onset of gastrulation. At this early stage, Shp2 mutant cells collect in the primitive streak of the epiblast and thus show deficiencies in their contribution to the mesoderm lineage. In high-contribution chimeras, we show that overaccumulation of Shp2 mutant cells at the posterior end of the embryo results in two abnormal phenotypes: spina bifida and secondary neural tubes. Consistent with a failure to undergo morphogenic movements at gastrulation, Shp2 is required for embryo fibroblast cells to mount a positive chemotactic response to acidic fibroblast growth factor in vitro. Our results demonstrate that Shp2 is required at the initial steps of gastrulation, as nascent mesodermal cells form and migrate away from the primitive streak. The aberrant behavior of Shp2 mutant cells at gastrulation may result from their inability to properly respond to signals initiated by fibroblast growth factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.