ArticleProceedings of the National Academy of Sciences of the United States of America1999
Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 90 citations in OpenAlex.
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.Nature communications · 2026Article
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.bioRxiv : the preprint server for biology · 2026Article
- Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy.Bio-protocol · 2021Article
- Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.Current biology : CB · 2019Article
- Architecture of the CBF3-centromere complex of the budding yeast kinetochore.Nature structural & molecular biology · 2018Article
- Fork pausing allows centromere DNA loop formation and kinetochore assembly.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.Cell reports · 2018Article
- Studying protein-DNA interactions using atomic force microscopy.Seminars in cell & developmental biology · 2018Review
- RNA helicase A activity is inhibited by oncogenic transcription factor EWS-FLI1.Nucleic acids research · 2015Article
- DNA G-segment bending is not the sole determinant of topology simplification by type II DNA topoisomerases.Scientific reports · 2014Article
- Review
- Spatial rule-based modeling: a method and its application to the human mitotic kinetochore.Cells · 2013Article
- The unconventional structure of centromeric nucleosomes.Chromosoma · 2012Review
- Mechanisms of unphosphorylated STAT3 transcription factor binding to DNA.The Journal of biological chemistry · 2012Article
- Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.The Journal of biological chemistry · 2012Article
- Tripartite organization of centromeric chromatin in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Functional centromeres determine the activation time of pericentric origins of DNA replication in Saccharomyces cerevisiae.PLoS genetics · 2012Article
- Nonhistone Scm3 binds to AT-rich DNA to organize atypical centromeric nucleosome of budding yeast.Molecular cell · 2011Article
- The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A differential association of Apolipoprotein E isoforms with the amyloid-β oligomer in solution.Proteins · 2011Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Yeast centromeric DNA (CEN DNA) binding factor 3 (CBF3) is a multisubunit protein complex that binds to the essential CDEIII element in CEN DNA. The four CBF3 proteins are required for accurate chromosome segregation and are considered to be core components of the yeast kinetochore. We have examined the structure of the CBF3-CEN DNA complex by atomic force microscopy. Assembly of CBF3-CEN DNA complexes was performed by combining purified CBF3 proteins with a DNA fragment that includes the CEN region from yeast chromosome III. Atomic force microscopy images showed DNA molecules with attached globular bodies. The contour length of the DNA containing the complex is approximately 9% shorter than the DNA alone, suggesting some winding of DNA within the complex. The measured location of the single binding site indicates that the complex is located asymmetrically to the right of CDEIII extending away from CDEI and CDEII, which is consistent with previous data. The CEN DNA is bent approximately 55 degrees at the site of complex formation. A significant fraction of the complexes are linked in pairs, showing three to four DNA arms, with molecular volumes approximately three times the mean volumes of two-armed complexes. These multi-armed complexes indicate that CBF3 can bind two DNA molecules together in vitro and, thus, may be involved in holding together chromatid pairs during mitosis.
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