ArticleProceedings of the National Academy of Sciences of the United States of America1999
Raft association of SNAP receptors acting in apical trafficking in Madin-Darby canine kidney cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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81 citing papers in PubMed.
- Syntaxin-3 Regulates Tight Junction Assembly in Human Retinal Pigment Epithelium.Cell biochemistry and function · 2026Article
- Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties.bioRxiv : the preprint server for biology · 2025Article
- Article
- Membrane lipid rafts are required for AMPA receptor tyrosine phosphorylation.Frontiers in synaptic neuroscience · 2022Article
- The ins and outs of the Arf4-based ciliary membrane-targeting complex.Small GTPases · 2021Review
- Axon growth and synaptic function: A balancing act for axonal regeneration and neuronal circuit formation in CNS trauma and disease.Developmental neurobiology · 2020Review
- STX4 expression is associated with classification, clinical stage and lymphatic metastasis in ovarian cancer.Translational cancer research · 2019Article
- An interaction network between the SNARE VAMP7 and Rab GTPases within a ciliary membrane-targeting complex.Journal of cell science · 2018Article
- Biomechanical Control of Lysosomal Secretion Via the VAMP7 Hub: A Tug-of-War between VARP and LRRK1.iScience · 2018Article
- Hepatitis C Virus Induces the Localization of Lipid Rafts to Autophagosomes for Its RNA Replication.Journal of virology · 2017Article
- Abnormal Rab11-Rab8-vesicles cluster in enterocytes of patients with microvillus inclusion disease.Traffic (Copenhagen, Denmark) · 2017Article
- Organization and dynamics of SNARE proteins in the presynaptic membrane.Frontiers in physiology · 2015Review
- Milk secretion: The role of SNARE proteins.Journal of mammary gland biology and neoplasia · 2014Review
- Drosophila SNAP-29 is an essential SNARE that binds multiple proteins involved in membrane traffic.PloS one · 2014Article
- VAMP7 modulates ciliary biogenesis in kidney cells.PloS one · 2014Article
- Syntaxin-3 regulates newcomer insulin granule exocytosis and compound fusion in pancreatic beta cells.Diabetologia · 2013Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Annexin A2 is involved in the formation of hepatitis C virus replication complex on the lipid raft.Journal of virology · 2012Article
- Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function.Molecular biology of the cell · 2011Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the relationships between the apical sorting mechanism using lipid rafts and the soluble N-ethyl maleimide-sensitive factor attachment protein receptor (SNARE) machinery, which is involved in membrane docking and fusion. We first confirmed that anti-alpha-SNAP antibodies inhibit the apical pathway in Madin- Darby canine kidney (MDCK) cells; in addition, we report that a recombinant SNAP protein stimulates the apical transport whereas a SNAP mutant inhibits this transport step. Based on t-SNARE overexpression experiments and the effect of botulinum neurotoxin E, syntaxin 3 and SNAP-23 have been implicated in apical membrane trafficking. Here, we show in permeabilized MDCK cells that antisyntaxin 3 and anti-SNAP-23 antibodies lower surface delivery of an apical reporter protein. Moreover, using a similar approach, we show that tetanus toxin-insensitive, vesicle-associated membrane protein (TI-VAMP; also called VAMP7), a recently described apical v-SNARE, is involved. Furthermore, we show the presence of syntaxin 3 and TI-VAMP in isolated apical carriers. Polarized apical sorting has been postulated to be mediated by the clustering of apical proteins into dynamic sphingolipid-cholesterol rafts. We provide evidence that syntaxin 3 and TI-VAMP are raft-associated. These data support a raft-based mechanism for the sorting of not only apically destined cargo but also of SNAREs having functions in apical membrane-docking and fusion events.
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