ArticleProceedings of the National Academy of Sciences of the United States of America1999
Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacterium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 79 citations in OpenAlex.
- CRISPR/Cas9 and Agrobacterium tumefaciens virulence proteins synergistically increase efficiency of precise genome editing via homology directed repair in plants.Journal of experimental botany · 2023Article
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Pathways of DNA Transfer to Plants fromAnnual review of phytopathology · 2019Article
- A Targeted and Stable Polymeric Nanoformulation Enhances Systemic Delivery of mRNA to Tumors.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- The chimeric multi-domain proteins mediating specific DNA transfer for hepatocellular carcinoma treatment.Cancer cell international · 2016Article
- Applying horizontal gene transfer phenomena to enhance non-viral gene therapy.Journal of controlled release : official journal of the Controlled Release Society · 2013Review
- The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.The International journal of developmental biology · 2013Review
- Delivering factors for reprogramming a somatic cell to pluripotency.International journal of stem cells · 2012Review
- A novel method of transgene delivery into triticale plants using the Agrobacterium transferred DNA-derived nano-complex.Plant physiology · 2012Article
- New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation.The EMBO journal · 2010Review
- Article
- Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus.Microbial biotechnology · 2009Review
- Yeast-plant coupled vector system for identification of nuclear proteins.Plant physiology · 2007Article
- Agrobacterium proteins VirD2 and VirE2 mediate precise integration of synthetic T-DNA complexes in mammalian cells.EMBO reports · 2004Article
- Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool.Microbiology and molecular biology reviews : MMBR · 2003Review
- VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity.The EMBO journal · 2001Article
- Kinetics and mechanism of DNA uptake into the cell nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Genetic transformation of HeLa cells by Agrobacterium.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Import of Agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins.The Plant cell · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Import of DNA into mammalian nuclei is generally inefficient. Therefore, one of the current challenges in human gene therapy is the development of efficient DNA delivery systems. Here we tested whether bacterial proteins could be used to target DNA to mammalian cells. Agrobacterium tumefaciens, a plant pathogen, efficiently transfers DNA as a nucleoprotein complex to plant cells. Agrobacterium-mediated T-DNA transfer to plant cells is the only known example for interkingdom DNA transfer and is widely used for plant transformation. Agrobacterium virulence proteins VirD2 and VirE2 perform important functions in this process. We reconstituted complexes consisting of the bacterial virulence proteins VirD2, VirE2, and single-stranded DNA (ssDNA) in vitro. These complexes were tested for import into HeLa cell nuclei. Import of ssDNA required both VirD2 and VirE2 proteins. A VirD2 mutant lacking its C-terminal nuclear localization signal was deficient in import of the ssDNA-protein complexes into nuclei. Import of VirD2-ssDNA-VirE2 complexes was fast and efficient, and was shown to depended on importin alpha, Ran, and an energy source. We report here that the bacterium-derived and plant-adapted protein-DNA complex, made in vitro, can be efficiently imported into mammalian nuclei following the classical importin-dependent nuclear import pathway. This demonstrates the potential of our approach to enhance gene transfer to animal cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.