ArticleBiophysical journal1999
High-speed, random-access fluorescence microscopy: II. Fast quantitative measurements with voltage-sensitive dyes.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 112 citations in OpenAlex.
- Monitoring presynaptic calcium dynamics with membrane-impermeant fluorescent indicators in motor nerve endings.Biophysical reviews · 2025Review
- Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging.Scientific reports · 2024Article
- Label-free, non-contact determination of resting membrane potential using dielectrophoresis.Scientific reports · 2024Article
- Simulating the Voltage-Dependent Fluorescence of Di-8-ANEPPS in a Lipid Membrane.The journal of physical chemistry letters · 2023Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- Recent progress in optical voltage-sensor technology and applications to cardiac research: from single cells to whole hearts.Progress in biophysics and molecular biology · 2020Review
- Comprehensive Imaging of Sensory-Evoked Activity of Entire Neurons Within the Awake Developing Brain Using Ultrafast AOD-Based Random-Access Two-Photon Microscopy.Frontiers in neural circuits · 2020Article
- Erythrocyte plasma membrane potential: past and current methods for its measurement.Biophysical reviews · 2019Review
- Article
- Red Fluorescent Genetically Encoded Voltage Indicators with Millisecond Responsiveness.Sensors (Basel, Switzerland) · 2019Article
- Review
- Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties.Scientific reports · 2018Article
- Will big data yield new mathematics? An evolving synergy with neuroscience.IMA journal of applied mathematics · 2016Article
- Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS.The Journal of general physiology · 2013Article
- Action potential propagation in transverse-axial tubular system is impaired in heart failure.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation.IEEE transactions on bio-medical engineering · 2011Article
- Electro-optical imaging microscopy of dye-doped artificial lipidic membranes.Biophysical journal · 2009Article
- Chemosensory responses to CO2 in multiple brain stem nuclei determined using a voltage-sensitive dye in brain slices from rats.Journal of neurophysiology · 2009Article
- Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes.Biophysical journal · 2009Review
- Uniform action potential repolarization within the sarcolemma of in situ ventricular cardiomyocytes.Biophysical journal · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
An improved method for making fast quantitative determinations of membrane potential with voltage-sensitive dyes is presented. This method incorporates a high-speed, random-access, laser-scanning scheme (Bullen et al., 1997. Biophys. J. 73:477-491) with simultaneous detection at two emission wavelengths. The basis of this ratiometric approach is the voltage-dependent shift in the emission spectrum of the voltage-sensitive dye di-8-butyl-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (di-8-ANEPPS). Optical measurements are made at two emission wavelengths, using secondary dichroic beamsplitting and dual photodetectors (<570 nm and >570 nm). Calibration of the ratiometric measurements between signals at these wavelengths was achieved using simultaneous optical and patch-clamp measurements from adjacent points. Data demonstrating the linearity, precision, and accuracy of this technique are presented. Records obtained with this method exhibited a voltage resolution of approximately 5 mV, without any need for temporal or spatial averaging. Ratiometric recordings of action potentials from isolated hippocampal neurons are used to illustrate the usefulness of this approach. This method is unique in that it is the first to allow quantitative determination of dynamic membrane potential changes in a manner optimized for both high spatiotemporal resolution (2 micrometers and <0.5 ms) and voltage discrimination.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.