ArticleBiophysical journal1999
Thiol oxidation of actin produces dimers that enhance the elasticity of the F-actin network.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Bench evidence, bedside uncertainty: hyperoxia, mechanical ventilation and lung injury.European respiratory review : an official journal of the European Respiratory Society · 2026Review
- Morphological control of bundled actin networks subject to fixed-mass depletion.The Journal of chemical physics · 2024Article
- Disulfidptosis, A Novel Cell Death Pathway: Molecular Landscape and Therapeutic Implications.Aging and disease · 2024Review
- Dithiothreitol Affects the Fertilization Response in Immature and Maturing Starfish Oocytes.Biomolecules · 2023Article
- Hydrodynamic and Polyelectrolyte Properties of Actin Filaments: Theory and Experiments.Polymers · 2022Article
- Article
- Redox Regulation of the Actin Cytoskeleton in Cell Migration and Adhesion: On the Way to a Spatiotemporal View.Frontiers in cell and developmental biology · 2020Review
- Actin filaments-A target for redox regulation.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Using cytochalasins to improve current chemotherapeutic approaches.Anti-cancer agents in medicinal chemistry · 2015Article
- Reversibility and Viscoelastic Properties of Micropillar Supported and Oriented Magnesium Bundled F-Actin.PloS one · 2015Article
- Protein disulfide isomerase directly interacts with β-actin Cys374 and regulates cytoskeleton reorganization.The Journal of biological chemistry · 2014Article
- Surface contact stimulates the just-in-time deployment of bacterial adhesins.Molecular microbiology · 2012Article
- Extracellular inhibitors, repellents, and semaphorin/plexin/MICAL-mediated actin filament disassembly.Cytoskeleton (Hoboken, N.J.) · 2011Review
- Redox control of vascular smooth muscle migration.Antioxidants & redox signaling · 2010Review
- Structure and activity of the axon guidance protein MICAL.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Ultrafine particles cause cytoskeletal dysfunctions in macrophages: role of intracellular calcium.Particle and fibre toxicology · 2005Article
- Estrogen, mitochondria, and growth of cancer and non-cancer cells.Journal of carcinogenesis · 2005Article
- The mechanics of F-actin microenvironments depend on the chemistry of probing surfaces.Biophysical journal · 2000Article
- Mechanics of living cells measured by laser tracking microrheology.Biophysical journal · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Slow oxidation of sulfhydryls, forming covalently linked actin dimers and higher oligomers, accounts for increases in the shear elasticity of purified actin observed after aging. Disulfide-bonded actin dimers are incorporated into F-actin during polymerization and generate cross-links between actin filaments. The large gel strength of oxidized actin (>100 Pa for 1 mg/ml) in the absence of cross-linking proteins falls to within the theoretically predicted order of magnitude for uncross-linked actin filament networks (1 Pa) with the addition of sufficient concentrations of reducing agents such as 5 mM dithiothreitol or 10 mM beta-mercaptoethanol. As little as 1 gelsolin/1000 actin subunits also lowers the high storage modulus of oxidized actin. The effects of gelsolin may be both to increase filament number as it severs F-actin and to cover the barbed end of an actin filament, which otherwise might cross-link to the side of another filament via an actin dimer. These new findings may explain why previous studies of actin rheology report a wide range of values when purified actin is polymerized without added regulatory proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.