ArticleBiophysical journal1999
Time courses of mammalian cell electropermeabilization observed by millisecond imaging of membrane property changes during the pulse.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 153 citations in OpenAlex.
- Article
- Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro.Molecules (Basel, Switzerland) · 2021Article
- Electroporation-Based Treatments in Urology.Cancers · 2020Review
- Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of CaInternational journal of molecular sciences · 2020Article
- Nanovolcano microelectrode arrays: toward long-term on-demand registration of transmembrane action potentials by controlled electroporation.Microsystems & nanoengineering · 2020Article
- A Review on Electroporation-Based Intracellular Delivery.Molecules (Basel, Switzerland) · 2018Review
- Spatio-temporal dynamics of calcium electrotransfer during cell membrane permeabilization.Drug delivery and translational research · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Control by Low Levels of Calcium of Mammalian Cell Membrane Electropermeabilization.The Journal of membrane biology · 2018Article
- Asymmetric Patterns of Small Molecule Transport After Nanosecond and Microsecond Electropermeabilization.The Journal of membrane biology · 2018Article
- Continuous and High-Throughput Electromechanical Lysis of Bacterial Pathogens Using Ion Concentration Polarization.Analytical chemistry · 2018Article
- Electropermeabilization of Inner and Outer Cell Membranes with Microsecond Pulsed Electric Fields: Quantitative Study with Calcium Ions.Scientific reports · 2017Article
- Measuring the potential energy barrier to lipid bilayer electroporation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Theoretical Study of Molecular Transport Through a Permeabilized Cell Membrane in a Microchannel.The Journal of membrane biology · 2017Article
- Dielectrophoresis study of temporal change in internal conductivity of single CHO cells after electroporation by pulsed electric fields.Biomicrofluidics · 2017Article
- Dependence of Electroporation Detection Threshold on Cell Radius: An Explanation to Observations Non Compatible with Schwan's Equation Model.The Journal of membrane biology · 2016Article
- Micro- and Nanoscale Technologies for Delivery into Adherent Cells.Trends in biotechnology · 2016Review
- Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC³s).Micromachines · 2016Review
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Effects of nanosecond pulse electric fields on cellular elasticity.Micron (Oxford, England : 1993) · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time courses of electropermeabilization were analyzed during the electric field application using a rapid fluorescent imaging system. Exchanges of calcium ions through electropermeabilized membrane of Chinese hamster ovary cells were found to be asymmetrical. Entry of calcium ions during a millisecond pulse occurred on the anode-facing cell hemisphere. Entry through the region facing the cathode was observed only after the pulse. Leakage of intracellular calcium ions from electropermeabilized cell in low-calcium content medium was observed only from the anode-facing side. The exchanges during the pulse were mostly due to diffusion-driven processes, i.e., governed by the concentration gradient. Interaction of propidium iodide, a dye sensitive to the structural alteration of membrane, with cell membrane was asymmetrical during electropermeabilization. Localized enhancement of the dye fluorescence was observed during and after the pulsation on the cell surface. Specific staining of a limited anode-facing part of the membrane was observed as soon as the pulse was applied. The membrane fluorescence level increased during and immediately after the pulse whereas the geometry of the staining was unchanged. The membrane regions stained by propidium iodide were the same as those where calcium exchanges occurred. The fraction of the membrane on which structural alterations occurred was defined by the field strength. The density of defects was governed by the pulse duration. Electropermeabilization is a localized but asymmetrical process. The membrane defects are created unequally on the two cell sides during the pulse, implying a vectorial effect of the electric field on the membrane.
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