ArticleBiophysical journal1999
Mucosal mast cell secretion processes imaged using three-photon microscopy of 5-hydroxytryptamine autofluorescence.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence.Journal of biophotonics · 2025Pooled it
- Hormonal contraceptives and the brain: A systematic review on 60 years of neuroimaging, EEG, and biochemical studies in humans and animals.Frontiers in neuroendocrinology · 2023Pooled it
- Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.Biomedical optics express · 2025Article
- Measurement of Exocytosis in Genetically Manipulated Mast Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Fluorescence of serotonin in the visible spectrum upon multiphotonic photoconversion.Biomedical optics express · 2020Article
- Molecular imaging with nanoparticles: the dwarf actors revisited 10 years later.Histochemistry and cell biology · 2018Review
- Label-free imaging of neurotransmitters in live brain tissue by multi-photon ultraviolet microscopy.Neuronal signaling · 2018Review
- Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc.Micromachines · 2016Article
- Action potential-independent and pharmacologically unique vesicular serotonin release from dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Insulin-containing lipogenic stimuli suppress mast cell degranulation potential and up-regulate lipid body biogenesis and eicosanoid secretion in a PPARγ-independent manner.Journal of leukocyte biology · 2012Article
- Spatiotemporal resolution of mast cell granule exocytosis reveals correlation with Ca2+ wave initiation.Journal of cell science · 2012Article
- Stimulus-dependent changes in optical responses of the dorsal cochlear nucleus using voltage-sensitive dye.Journal of neurophysiology · 2011Article
- The RBL-2H3 cell line: its provenance and suitability as a model for the mast cell.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2009Review
- Multiphoton microscopy of prostate and periprostatic neural tissue: a promising imaging technique for improving nerve-sparing prostatectomy.Journal of endourology · 2009Article
- Review
- Fusion gains independence.The Journal of general physiology · 2008Article
- Ca-dependent nonsecretory vesicle fusion in a secretory cell.The Journal of general physiology · 2008Article
- Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues.Biophysical journal · 2007Article
- Single- and multi-photon excited fluorescence from serotonin complexed with beta-cyclodextrin.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2006Article
- Interpreting second-harmonic generation images of collagen I fibrils.Biophysical journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The secretion process of the mucosal mast cell line RBL-2H3 was imaged using infrared three photon excitation (3PE) of serotonin (5-hydroxytryptamine, 5-HT) autofluorescence, a measurement previously difficult because of the technical intractability of deep UV optics. Images of prestimulation 5-HT distributions were analyzed in loaded cell populations (those incubated in a 5-HT-rich medium overnight) and in unloaded populations and were found to be strictly quantifiable by comparison with bulk population high-performance liquid chromatography measurements. Antigenically stimulated cells were observed to characteristically ruffle and spread as granular 5-HT disappeared with no detectable granule movement. Individual cells exhibited highly heterogeneous release kinetics, often with quasi-periodic bursts. Neighboring granule disappearances were correlated, indicative of either spatially localized signaling or granule-granule interactions. In one-half of the granule release events, weak residual fluorescence was visible suggestive of leftover 5-HT still bound to the granule matrix. The terminal stages of secretion (>300 s) consisted primarily of unresolved granules and remainder 5-HT leakage from already released granules.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.