ArticleAnalytical biochemistry1999
Lifetime-based pH sensors: indicators for acidic environments.
Article in Analytical biochemistry, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Assessing Perturbations in Lysosome Health by Flow Cytometry in iPSC-Derived Human Neuron Cultures.Methods in molecular biology (Clifton, N.J.) · 2026Article
- pH biosensing in the plant apoplast-a focus on root cell elongation.Plant physiology · 2021Review
- Improving the efficiency of CONature chemistry · 2021Article
- 5-Bromo-4',5'-bis(dimethylamino)fluorescein: Synthesis and Photophysical Studies.Molecules (Basel, Switzerland) · 2018Article
- A theoretical-experimental methodology for assessing the sensitivity of biomedical spectral imaging platforms, assays, and analysis methods.Journal of biophotonics · 2018Review
- Comparative Study of Poly (ε-Caprolactone) and Poly(Lactic-co-Glycolic Acid) -Based Nanofiber Scaffolds for pH-Sensing.Pharmaceutical research · 2016Article
- Versatile design of biohybrid light-harvesting architectures to tune location, density, and spectral coverage of attached synthetic chromophores for enhanced energy capture.Photosynthesis research · 2014Article
- Anilinomethylrhodamines: pH sensitive probes with tunable photophysical properties by substituent effect.The Journal of organic chemistry · 2013Article
- Tripping on acid: trans-kingdom perspectives on biological acids in immunity and pathogenesis.PLoS pathogens · 2013Review
- A multimode optical imaging system for preclinical applications in vivo: technology development, multiscale imaging, and chemotherapy assessment.Molecular imaging and biology · 2012Article
- FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator.Nanoscale research letters · 2011Article
- A series of naphthalimide derivatives as intra and extracellular pH sensors.Biomaterials · 2010Article
- Exploring the structure of the N-terminal domain of CP29 with ultrafast fluorescence spectroscopy.European biophysics journal : EBJ · 2010Article
- The spatially variable inhibition by water deficit of maize root growth correlates with altered profiles of proton flux and cell wall pH.Plant physiology · 2004Article
- Influence of structural variation on nuclear localization of DNA-binding polyamide-fluorophore conjugates.Nucleic acids research · 2004Article
- Fluorescence lifetime-resolved pH imaging of living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2003Article
- Fluorescence lifetime characterization of novel low-pH probes.Analytical biochemistry · 2001Article
- Fluorescence properties of albumin blue 633 and 670 in plasma and whole blood.Journal of biomedical optics · 2001Article
- The HPV16 E5 oncogene inhibits endocytic trafficking.Oncogene · 2000Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We characterized the pH-dependent intensity decays of three fluorophores, Oregon green 514 carboxylic acid, Cl-NERF, and DM-NERF, using frequency-domain fluorometry, with the objective of identifying lifetime-based sensors for low pH values. These three probes were originally designed as dual excitation wavelength-ratiometric probes, with high photostability and high quantum yields in aqueous solutions. We found that their fluorescence intensity decays were strongly dependent on pH. Moreover, global intensity decays analysis reveals that these probes have double exponential intensity decays at intermediate pH values and that the decay time amplitudes are greatly dependent on pH. The longer lifetime components originated from the unprotonated forms and the shorter components from the protonated forms. Both forms can emit fluorescence at intermediate pH values. The apparent pKa values were also determined from the titration curves of phase angles and modulations versus pH for the purpose of pH sensing. The apparent pKa values range from pH 3 to 5, a range where lifetime-based sensors are not presently reported. Since these probes show low pKa values and display substantial phase and modulation changes with pH, they are suitable as lifetime-based pH sensors to monitor the pH changes in acidic environments. One potential application of these probes is to trace the pH in different cellular compartments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.