ArticleJournal of bacteriology1999
Genes coding for phosphotransacetylase and acetate kinase in Sinorhizobium meliloti are in an operon that is inducible by phosphate stress and controlled by phoB.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- A description of Joostella sp. strain CR20 with potential biotechnological applications.Antonie van Leeuwenhoek · 2024Article
- Comparative Proteomic Analysis of Psychrophilic vs. Mesophilic Bacterial Species Reveals Different Strategies to Achieve Temperature Adaptation.Frontiers in microbiology · 2022Article
- CyanobacteriumBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Genome-Wide Metabolic Reconstruction of the Synthesis of Polyhydroxyalkanoates from Sugars and Fatty Acids byMicroorganisms · 2021Review
- Inorganic Phosphate Solubilization by Rhizosphere BacteriumFrontiers in microbiology · 2020Article
- Article
- The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism.Microbial cell factories · 2017Article
- Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice.Proteome science · 2012Article
- Differential proteomic analysis highlights metabolic strategies associated with balhimycin production in Amycolatopsis balhimycina chemostat cultivations.Microbial cell factories · 2010Article
- The linear chromosome of the plant-pathogenic mycoplasma 'Candidatus Phytoplasma mali'.BMC genomics · 2008Article
- Fine-tuning of galactoglucan biosynthesis in Sinorhizobium meliloti by differential WggR (ExpG)-, PhoB-, and MucR-dependent regulation of two promoters.Journal of bacteriology · 2008Article
- Cloning, expression, and characterization of an acetate kinase from a high rate of biohydrogen bacterial strain Ethanoligenens sp. hit B49.Current microbiology · 2007Article
- Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures.Extremophiles : life under extreme conditions · 2007Article
- Regulation and properties of PstSCAB, a high-affinity, high-velocity phosphate transport system of Sinorhizobium meliloti.Journal of bacteriology · 2006Article
- Genome prediction of PhoB regulated promoters in Sinorhizobium meliloti and twelve proteobacteria.Nucleic acids research · 2006Article
- Unraveling the secret lives of bacteria: use of in vivo expression technology and differential fluorescence induction promoter traps as tools for exploring niche-specific gene expression.Microbiology and molecular biology reviews : MMBR · 2005Review
- The acetate switch.Microbiology and molecular biology reviews : MMBR · 2005Review
- Global transcriptional analysis of the phosphate starvation response in Sinorhizobium meliloti strains 1021 and 2011.Molecular genetics and genomics : MGG · 2004Article
- Development and application of a dapB-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium Pseudomonas stutzeri A15.Applied and environmental microbiology · 2003Article
- The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent work in this laboratory has shown that the gene coding for acetate kinase (ackA) in Sinorhizobium meliloti is up-regulated in response to phosphate limitation. Characterization of the region surrounding ackA revealed that it is adjacent to pta, which codes for phosphotransacetylase, and that these two genes are part of an operon composed of at least two additional genes in the following order: an open reading frame (orfA), pta, ackA, and the partial sequence of a gene with an inferred peptide that has a high degree of homology to enoyl-ACP reductase (fabI). Experiments combining enzyme assays, a chromosomal lacZ::ackA transcriptional fusion, complementation analysis with cosmid subclones, and the creation of mutations in pta and ackA all indicated that the orfA-pta-ackA-fabI genes are cotranscribed in response to phosphate starvation. Primer extension was used to map the position of the phosphate starvation-inducible transcriptional start sites upstream of orfA. The start sites were found to be preceded by a sequence having similarity to PHO boxes from other phosphate-regulated genes in S. meliloti and to the consensus PHO box in Escherichia coli. Introduction of a phoB mutation in the wild-type strain eliminated elevated levels of acetate kinase and phosphotransacetylase activities in response to phosphate limitation and also eliminated the phosphate stress-induced up-regulation of the ackA::lacZ fusion. Mutations in either ackA alone or both pta and ackA did not affect the nodulation or nitrogen fixation phenotype of S. meliloti.
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