ArticleJournal of bacteriology1999
The acid-inducible asr gene in Escherichia coli: transcriptional control by the phoBR operon.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 83 citations in OpenAlex.
- SamT, a novel peptide modulator of a two-component system revealed by the specific activation of a small RNA in Enterobacteriaceae.Nucleic acids research · 2026Article
- Synergistic attenuation of Aeromonas dhakensis virulence by dual deletion of PhoBR and KdpE two-component systems and its protective immunity in zebrafish.Applied microbiology and biotechnology · 2026Article
- Toehold Switch-Based Approach for Engineering Acid-Tolerance Modules to Enhance Production Robustness of Industrial E. coli Strains at Low pH.Microbial biotechnology · 2025Article
- High efficiency rare earth element bioleaching with systems biology guided engineering of Gluconobacter oxydans.Communications biology · 2025Article
- Article
- Dual ICA to extract interacting sets of genes and conditions from transcriptomic data.ACM-BCB ... ... : the ... ACM Conference on Bioinformatics, Computational Biology and Biomedicine. ACM Conference on Bioinformatics, Computational Biology and Biomedicine · 2023Article
- Article
- Genome-wide mapping of thebioRxiv : the preprint server for biology · 2023Article
- Intracellular acidification is a hallmark of thymineless death in E. coli.PLoS genetics · 2022Article
- Synthetic acid stress-tolerance modules improve growth robustness and lysine productivity of industrial Escherichia coli in fermentation at low pH.Microbial cell factories · 2022Article
- PhoPR Contributes to Staphylococcus aureus Growth during Phosphate Starvation and Pathogenesis in an Environment-Specific Manner.Infection and immunity · 2018Article
- Article
- Transient MutS-Based Hypermutation System for Adaptive Evolution of Lactobacillus casei to Low pH.Applied and environmental microbiology · 2017Article
- The Pho regulon: a huge regulatory network in bacteria.Frontiers in microbiology · 2015Review
- PhoB activates Escherichia coli O157:H7 virulence factors in response to inorganic phosphate limitation.PloS one · 2014Article
- Regulation Systems of Bacteria such as Escherichia coli in Response to Nutrient Limitation and Environmental Stresses.Metabolites · 2013Article
- Genetic diversity in cultured and wild marine cyanomyoviruses reveals phosphorus stress as a strong selective agent.The ISME journal · 2013Article
- Metabolic Regulation of a Bacterial Cell System with Emphasis on Escherichia coli Metabolism.ISRN biochemistry · 2013Review
- Gene expression analysis of Salmonella enterica Enteritidis Nal(R) and Salmonella enterica Kentucky 3795 exposed to HCl and acetic acid in rich medium.Foodborne pathogens and disease · 2012Article
- Characterization of Escherichia coli dinJ-yafQ toxin-antitoxin system using insights from mutagenesis data.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli responds to external acidification (pH 4.0 to 5.0) by synthesizing a newly identified, approximately 450-nucleotide RNA component. At maximal levels of induction it is one of the most abundant small RNAs in the cell and is relatively stable bacterial RNA. The acid-inducible RNA was purified, and the gene encoding it, designated asr (for acid shock RNA), mapped at 35.98 min on the E. coli chromosome. Analysis of the asr DNA sequence revealed an open reading frame coding for a 111-amino-acid polypeptide with a deduced molecular mass of approximately 11.6 kDa. According to computer-assisted analysis, the predicted polypeptide contains a typical signal sequence of 30 amino acids and might represent either a periplasmic or an outer membrane protein. The asr gene cloned downstream from a T7 promoter was translated in vivo after transcription using a T7 RNA polymerase transcription system. Expression of a plasmid-encoded asr::lacZ fusion under a native asr promoter was reduced approximately 15-fold in a complex medium, such as Luria-Bertani medium, versus the minimal medium. Transcription of the chromosomal asr was abolished in the presence of a phoB-phoR (a two-component regulatory system, controlling the pho regulon inducible by phosphate starvation) deletion mutant. Acid-mediated induction of the asr gene in the Delta(phoB-phoR) mutant strain was restored by introduction of the plasmid with cloned phoB-phoR genes. Primer extension analysis of the asr transcript revealed a region similar to the Pho box (the consensus sequence found in promoters transcriptionally activated by the PhoB protein) upstream from the determined transcription start. The asr promoter DNA region was demonstrated to bind PhoB protein in vitro. We discuss our results in terms of how bacteria might employ the phoB-phoR regulatory system to sense an external acidity and regulate transcription of the asr gene.
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