ArticleJournal of bacteriology1999
Regulation of expression of the Lactococcus lactis histidine operon.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Evolution of transcriptional regulation of histidine metabolism in Gram-positive bacteria.BMC genomics · 2022Pooled it
- Multi-omics Approach Reveals How Yeast Extract Peptides Shape Streptococcus thermophilus Metabolism.Applied and environmental microbiology · 2020Article
- Article
- ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403.Applied microbiology and biotechnology · 2015Article
- The T box riboswitch: A novel regulatory RNA that utilizes tRNA as its ligand.Biochimica et biophysica acta · 2014Review
- The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics.Molecular & cellular proteomics : MCP · 2013Article
- The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus durans is transcriptionally regulated by tyrosine concentration and extracellular pH.BMC microbiology · 2012Article
- NoxE NADH oxidase and the electron transport chain are responsible for the ability of Lactococcus lactis to decrease the redox potential of milk.Applied and environmental microbiology · 2010Article
- The T box mechanism: tRNA as a regulatory molecule.FEBS letters · 2010Review
- Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.Microbiology and molecular biology reviews : MMBR · 2009Review
- The D-2-hydroxyacid dehydrogenase incorrectly annotated PanE is the sole reduction system for branched-chain 2-keto acids in Lactococcus lactis.Journal of bacteriology · 2009Article
- High-throughput identification and validation of in situ-expressed genes of Lactococcus lactis.Applied and environmental microbiology · 2008Article
- An in silico analysis of T-box regulated genes and T-box evolution in prokaryotes, with emphasis on prediction of substrate specificity of transporters.BMC genomics · 2008Article
- Comparative genomic analysis of T-box regulatory systems in bacteria.RNA (New York, N.Y.) · 2008Article
- Luciferase detection during stationary phase in Lactococcus lactis.Applied and environmental microbiology · 2007Article
- Inference from proteobacterial operons shows piecewise organization: a reply to Price et al.Journal of molecular evolution · 2006Article
- Proteomic signature of Lactococcus lactis NCDO763 cultivated in milk.Applied and environmental microbiology · 2005Article
- Alternative lactose catabolic pathway in Lactococcus lactis IL1403.Applied and environmental microbiology · 2005Article
- Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403.Journal of bacteriology · 2005Article
- Fructose utilization in Lactococcus lactis as a model for low-GC gram-positive bacteria: its regulator, signal, and DNA-binding site.Journal of bacteriology · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Lactococcus lactis, the his operon contains all the genes necessary for histidine biosynthesis. It is transcribed from a unique promoter, localized 300 bp upstream of the first gene. The region corresponding to the untranslated 5' end of the transcript, named the his leader region, displays the typical features of the T box transcriptional attenuation mechanism which is involved in the regulation of many amino acid biosynthetic operons and tRNA synthetase genes in gram-positive bacteria. Here we describe the regulation of transcription of the his operon by the level of histidine in the growth medium. In the absence of histidine, two transcripts are present. One covers the entire operon, while the other stops at a terminator situated about 250 bp downstream of the transcription start point. DNA sequences implicated in regulation of the his operon were identified by transcriptional fusion with luciferase genes and site-directed mutagenesis. In addition to the previously defined sequences necessary for effective T-box-mediated regulation, new essential regions were identified. Eighteen percent of the positions of the his leader region were found to differ in seven distantly related strains of L. lactis. Analysis of the variable positions supports the folding model of the central part of the his leader region. Lastly, in addition to the T-box-mediated regulation, the operon is regulated at the level of initiation of transcription, which is repressed in the presence of histidine. An operator site, necessary for full repression, overlaps the terminator involved in the T box attenuation mechanism. The functionality of the operator is altered on plasmids with low and high copy numbers, suggesting that supercoiling may play a role in the expression of the his operon. The extents of regulation at the levels of initiation and attenuation of transcription are 6- to 8-fold and 14-fold, respectively. Together, the two levels of control allow a 120-fold range of regulation of the L. lactis operon by histidine.
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