ArticleJournal of bacteriology1999
Chromosome methylation and measurement of faithful, once and only once per cell cycle chromosome replication in Caulobacter crescentus.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 80 citations in OpenAlex.
- Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus.Current biology : CB · 2025Article
- Three factors ParA, TipN, and DnaA-mediated chromosome replication initiation are contributors of centromere segregation inMolecular biology of the cell · 2024Article
- TipN's involvement with centromere segregation in Caulobacter crescentus.bioRxiv : the preprint server for biology · 2023Article
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- Proteolysis dependent cell cycle regulation in Caulobacter crescentus.Cell division · 2022Review
- Activation of a signaling pathway by the physical translocation of a chromosome.Developmental cell · 2021Article
- Transcriptional rewiring of the GcrA/CcrM bacterial epigenetic regulatory system in closely related bacteria.PLoS genetics · 2021Article
- Characterization of the Chromosome Dimer Resolution Site in Caulobacter crescentus.Journal of bacteriology · 2019Article
- Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus.iScience · 2018Article
- Replicate Once Per Cell Cycle: Replication Control of Secondary Chromosomes.Frontiers in microbiology · 2018Review
- Novel Chromosome Organization Pattern inmBio · 2017Article
- The Caulobacter crescentus Homolog of DnaA (HdaA) Also Regulates the Proteolysis of the Replication Initiator Protein DnaA.Journal of bacteriology · 2015Article
- Article
- Nutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaA.PLoS genetics · 2015Article
- Redefining bacterial origins of replication as centralized information processors.Frontiers in microbiology · 2015Review
- The functions of DNA methylation by CcrM in Caulobacter crescentus: a global approach.Nucleic acids research · 2014Article
- Global methylation state at base-pair resolution of the Caulobacter genome throughout the cell cycle.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The β-sliding clamp directs the localization of HdaA to the replisome in Caulobacter crescentus.Microbiology (Reading, England) · 2013Article
- Article
- DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus.Molecular microbiology · 2013Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Caulobacter crescentus exhibits cell-type-specific control of chromosome replication and DNA methylation. Asymmetric cell division yields a replicating stalked cell and a nonreplicating swarmer cell. The motile swarmer cell must differentiate into a sessile stalked cell in order to replicate and execute asymmetric cell division. This program of cell division implies that chromosome replication initiates in the stalked cell only once per cell cycle. DNA methylation is restricted to the predivisional cell stage, and since DNA synthesis produces an unmethylated nascent strand, late DNA methylation also implies that DNA near the replication origin remains hemimethylated longer than DNA located further away. In this report, both assumptions are tested with an engineered Tn5-based transposon, Tn5Omega-MP. This allows a sensitive Southern blot assay that measures fully methylated, hemimethylated, and unmethylated DNA duplexes. Tn5Omega-MP was placed at 11 sites around the chromosome and it was clearly demonstrated that Tn5Omega-MP DNA near the replication origin remained hemimethylated longer than DNA located further away. One Tn5Omega-MP placed near the replication origin revealed small but detectable amounts of unmethylated duplex DNA in replicating stalked cells. Extra DNA synthesis produces a second unmethylated nascent strand. Therefore, measurement of unmethylated DNA is a critical test of the "once and only once per cell cycle" rule of chromosome replication in C. crescentus. Fewer than 1 in 1,000 stalked cells prematurely initiate a second round of chromosome replication. The implications for very precise negative control of chromosome replication are discussed with respect to the bacterial cell cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.