ArticleRNA (New York, N.Y.)1999
The cis acting sequences responsible for the differential decay of the unstable MFA2 and stable PGK1 transcripts in yeast include the context of the translational start codon.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 93 citations in OpenAlex.
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- Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability.bioRxiv : the preprint server for biology · 2023Article
- Controlling gene expression with deep generative design of regulatory DNA.Nature communications · 2022Article
- Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiation.Cell systems · 2022Article
- Increased expression of the fluorescent reporter protein ymNeonGreen inBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Disrupting upstream translation in mRNAs is associated with human disease.Nature communications · 2021Article
- eIF1 discriminates against suboptimal initiation sites to prevent excessive uORF translation genome-wide.RNA (New York, N.Y.) · 2020Article
- Inverted translational control of eukaryotic gene expression by ribosome collisions.PLoS biology · 2019Article
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- Cytoplasmic Amplification of Transcriptional Noise Generates Substantial Cell-to-Cell Variability.Cell systems · 2018Article
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- Tma64/eIF2D, Tma20/MCT-1, and Tma22/DENR Recycle Post-termination 40S Subunits In Vivo.Molecular cell · 2018Article
- The Interplay between the RNA Decay and Translation Machinery in Eukaryotes.Cold Spring Harbor perspectives in biology · 2018Review
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- Multiplexed gene control reveals rapid mRNA turnover.Science advances · 2017Article
- Analysis of the association between codon optimality and mRNA stability in Schizosaccharomyces pombe.BMC genomics · 2016Article
- Article
- Article
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2 authors at 1 institution in 1 country.
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Abstract
A general pathway of mRNA turnover has been described for yeast in which the 3' poly(A) tail is first deadenylated to an oligo(A) length, leading to decapping and subsequent 5'-3' exonucleolytic decay. The unstable MFA2 mRNA and the stable PGK1 mRNAs both decay through this pathway, albeit at different rates of deadenylation and decapping. To determine the regions of the mRNAs that are responsible for these differences, we examined the decay of chimeric mRNAs derived from the 5' untranslated, coding, and 3' untranslated regions of these two mRNAs. These experiments have led to the identification of the features of these mRNAs that lead to their different stabilities. The MFA2 mRNA is unstable solely because its 3' UTR promotes the rates of deadenylation and decapping; all other features of this mRNA are neutral with respect to mRNA decay rates. The PGK1 mRNA is stable because the sequence context of the PGK1 translation start codon and the coding region function together to stabilize the transcript, whereas the PGK13' UTR is neutral with respect to decay. Importantly, changes in the PGK1 start codon context that destabilized the transcript also reduced its translational efficiency. This observation suggests that the nature of the translation initiation complex modulates the rates of mRNA decapping and decay.
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