ArticleGenes & development1999
Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- Short-term hypoxia triggers ROS and SAFB mediated nuclear matrix and mRNA splicing remodeling.Redox biology · 2022Article
- BCL2-associated athanogene 6 exon24 contributes to testosterone synthesis and male fertility in mammals.Cell proliferation · 2022Article
- Structure, Activity and Function of the PRMT2 Protein Arginine Methyltransferase.Life (Basel, Switzerland) · 2021Review
- SRSF10: an atypical splicing regulator with critical roles in stress response, organ development, and viral replication.RNA (New York, N.Y.) · 2021Review
- Srrm234, but not canonical SR and hnRNP proteins, drive inclusion ofRNA (New York, N.Y.) · 2019Article
- SRSF2 Is Essential for Hematopoiesis, and Its Myelodysplastic Syndrome-Related Mutations Dysregulate Alternative Pre-mRNA Splicing.Molecular and cellular biology · 2015Article
- Concentration and Localization of Coexpressed ELAV/Hu Proteins Control Specificity of mRNA Processing.Molecular and cellular biology · 2015Article
- SR proteins control a complex network of RNA-processing events.RNA (New York, N.Y.) · 2015Article
- Article
- Aberrant splicing of HTT generates the pathogenic exon 1 protein in Huntington disease.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown.PLoS genetics · 2010Article
- Identification of small molecule and genetic modulators of AON-induced dystrophin exon skipping by high-throughput screening.PloS one · 2009Article
- Eye development under the control of SRp55/B52-mediated alternative splicing of eyeless.PloS one · 2007Article
- Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites.Molecular biology of the cell · 2006Article
- The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis.Molecular and cellular biology · 2005Article
- Regulation of alternative splicing by SRrp86 and its interacting proteins.Molecular and cellular biology · 2003Article
- Ectopic expression of atRSZ33 reveals its function in splicing and causes pleiotropic changes in development.Molecular biology of the cell · 2003Article
- A conserved Drosophila transportin-serine/arginine-rich (SR) protein permits nuclear import of Drosophila SR protein splicing factors and their antagonist repressor splicing factor 1.Molecular biology of the cell · 2002Article
- Regulation of alternative splicing by SRrp86 through coactivation and repression of specific SR proteins.RNA (New York, N.Y.) · 2002Article
- Dual function for U2AF(35) in AG-dependent pre-mRNA splicing.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specific recognition of splice sites within metazoan mRNA precursors (pre-mRNAs) is a potential stage for gene regulation by alternative splicing. Splicing factors of the SR protein family play a major role in this regulation, as they are required for early recognition of splice sites during spliceosome assembly. Here, we describe the characterization of RSF1, a splicing repressor isolated from Drosophila, that functionally antagonizes SR proteins. Like the latter, RSF1 comprises an amino-terminal RRM-type RNA-binding domain, whereas its carboxy-terminal part is enriched in glycine (G), arginine (R), and serine (S) residues (GRS domain). RSF1 induces a dose-sensitive inhibition of splicing for several reporter pre-mRNAs, an inhibition that occurs at the level of early splicing complexes formation. RSF1 interacts, through its GRS domain, with the RS domain of the SR protein SF2/ASF and prevents the latter from cooperating with the U1 small nuclear ribonucleoprotein particle (U1 snRNP) in binding pre-mRNA. Furthermore, overproduction of RSF 1 in the fly rescues several developmental defects caused by overexpression of the splicing activator SR protein B52/ SRp55. Therefore, RSF1 may correspond to the prototypical member of a novel family of general splicing repressors that selectively antagonize the effect of SR proteins on 5' splice-site recognition.
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