ArticleThe Journal of cell biology1999
Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed, 381 citations in OpenAlex.
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- Remyelination as a therapeutic target in the treatment of multiple sclerosis.AIMS neuroscience · 2025Review
- Sorting of complex sphingolipids within the cellular endomembrane systems.Frontiers in cell and developmental biology · 2024Review
- Rab3 mediates a pathway for endocytic sorting and plasma membrane recycling of ordered microdomains.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Phosphatidic Acid Accumulates at Areas of Curvature in Tubulated Lipid Bilayers and Liposomes.Biomolecules · 2022Article
- Structural basis for acyl chain control over glycosphingolipid sorting and vesicular trafficking.Cell reports · 2022Article
- Superelasticity of Plasma- and Synthetic Membranes Resulting from Coupling of Membrane Asymmetry, Curvature, and Lipid Sorting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Peptide-based supramolecular vaccine systems.Acta biomaterialia · 2021Review
- The composition and function ofmicroLife · 2021Article
- The inhibitory effect of ECG and EGCG dimeric procyanidins on colorectal cancer cells growth is associated with their actions at lipid rafts and the inhibition of the epidermal growth factor receptor signaling.Biochemical pharmacology · 2020Article
- Dual Gene Delivery Reagents From Antiproliferative Alkylphospholipids for Combined Antitumor Therapy.Frontiers in chemistry · 2020Article
- Induction of specific adaptive immune responses by immunization with newly designed artificial glycosphingolipids.Scientific reports · 2019Article
- Raman Spectroscopy Study of Curvature-Mediated Lipid Packing and Sorting in Single Lipid Vesicles.Biophysical journal · 2019Article
- Membrane curvature induces cardiolipin sorting.Communications biology · 2019Article
- Mucosal absorption of therapeutic peptides by harnessing the endogenous sorting of glycosphingolipids.eLife · 2018Article
- The glycolipid GM1 reshapes asymmetric biomembranes and giant vesicles by curvature generation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis.Journal of cell science · 2017Article
- Article
49 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in their alkyl tail length or unsaturation; these differences are expected to affect their distribution in membrane domains of varying fluidity or curvature. All three DiIs initially enter sorting endosomes containing endocytosed transferrin. DiIC16(3), with two long 16-carbon saturated tails is then delivered to late endosomes, whereas FAST DiI, with two cis double bonds in each tail, and DiIC12(3), with saturated but shorter (12-carbon) tails, are mainly found in the endocytic recycling compartment. We also find that DiOC16(3) (3,3'- dihexadecyloxacarbocyanine perchlorate) and FAST DiO (3, 3'-dilinoleyloxacarbocyanine perchlorate) behave similarly to their DiI counterparts. Furthermore, whereas a phosphatidylcholine analogue with a BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorophore attached at the end of a 5-carbon acyl chain is delivered efficiently to the endocytic recycling compartment, a significant fraction of another derivative with BODIPY attached to a 12-carbon acyl chain entered late endosomes. Our results thus suggest that endocytic organelles can sort membrane components efficiently based on their preference for association with domains of varying characteristics.
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