ArticleOncogene1999
The cyclin B2 promoter depends on NF-Y, a trimer whose CCAAT-binding activity is cell-cycle regulated.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 125 citations in OpenAlex.
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- Functional Analysis of Nuclear Factor Y in the Wing-Dimorphic Brown Planthopper,Frontiers in genetics · 2020Article
- The Antiproliferation Activity ofMycobiology · 2020Article
- The laminA/NF-Y protein complex reveals an unknown transcriptional mechanism on cell proliferation.Oncotarget · 2017Article
- NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and Proliferation in the Planarian Schmidtea mediterranea.PLoS genetics · 2016Article
- Context-specific role of SOX9 in NF-Y mediated gene regulation in colorectal cancer cells.Nucleic acids research · 2015Article
- E2F1-Mediated Induction of NFYB Attenuates Apoptosis via Joint Regulation of a Pro-Survival Transcriptional Program.PloS one · 2015Article
- iRegulon: from a gene list to a gene regulatory network using large motif and track collections.PLoS computational biology · 2014Article
- A systematic analysis of a mi-RNA inter-pathway regulatory motif.Journal of clinical bioinformatics · 2013Article
- Impaired cardiovascular function caused by different stressors elicits a common pathological and transcriptional response in zebrafish embryos.Zebrafish · 2013Article
- NF-YA underlies EZH2 upregulation and is essential for proliferation of human epithelial ovarian cancer cells.Molecular cancer research : MCR · 2013Article
- p53 can repress transcription of cell cycle genes through a p21(WAF1/CIP1)-dependent switch from MMB to DREAM protein complex binding at CHR promoter elements.Cell cycle (Georgetown, Tex.) · 2012Article
- Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Cyclin B2 is a regulator of p34cdc2 kinase, involved in G2/M progression of the cell cycle, whose gene is strictly regulated at the transcriptional level in cycling cells. The mouse promoter was cloned and three conserved CCAAT boxes were found. In this study, we analysed the mechanisms leading to activation of the cyclin B2 CCAAT boxes: a combination of (i) genomic footprinting, (ii) transfections with single, double and triple mutants, (iii) EMSAs with nuclear extracts, antibodies and NF-Y recombinant proteins and (iv) transfections with an NF-YA dominant negative mutant established the positive role of the three CCAAT sequences and proved that NF-Y plays a crucial role in their activation. NF-Y, an ubiquitous trimer containing histone fold subunits, activates several other promoters regulated during the cell cycle. To analyse the levels of NF-Y subunits in the different phases of the cycle, we separated MEL cells by elutriation, obtaining fractions >80% pure. The mRNA and protein levels of the histone-fold containing NF-YB and NF-YC were invariant, whereas the NF-YA protein, but not its mRNA, was maximal in mid-S and decreased in G2/M. EMSA confirmed that the CCAAT-binding activity followed the amount of NF-YA, indicating that this subunit is limiting within the NF-Y complex, and suggesting that post-transcriptional mechanisms regulate NF-YA levels. Our results support a model whereby fine tuning of this activator is important for phase-specific transcription of CCAAT-containing promoters.
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