ArticleThe Journal of cell biology1999
Dissection of cell division processes in the one cell stage Caenorhabditis elegans embryo by mutational analysis.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 173 citations in OpenAlex.
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- Germ fate determinants protect germ precursor cell division by reducing septin and anillin levels at the cell division plane.Molecular biology of the cell · 2024Article
- Ultrastructure Expansion Microscopy applied tomicroPublication biology · 2024Article
- Towards understanding centriole elimination.Open biology · 2023Review
- Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos.Genetics · 2023Article
- A spiral microfluidic device for rapid sorting, trapping, and long-term live imaging ofMicrosystems & nanoengineering · 2023Article
- Centrosomal enrichment and proteasomal degradation of SYS-1/β-catenin requires the microtubule motor dynein.Molecular biology of the cell · 2022Article
- Functional midbody assembly in the absence of a central spindle.The Journal of cell biology · 2022Article
- Review
- Visualizing the Dynamics of Cell Division by Live Imaging Drosophila Larval Brain Squashes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections.G3 (Bethesda, Md.) · 2021Article
- An RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans reveals the involvement of unexpected processes.G3 (Bethesda, Md.) · 2021Article
- Mechanistic insights into central spindle assembly mediated by the centralspindlin complex.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Quantifying Intracellular Particle Flows by DIC Object Tracking.Biophysical journal · 2021Article
- Centrosome Aurora A regulates RhoGEF ECT-2 localisation and ensures a single PAR-2 polarity axis inDevelopment (Cambridge, England) · 2019Article
- Review
- The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge.Journal of cell science · 2019Article
- Mitotic Cell Division inGenetics · 2019Review
20 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify novel components required for cell division processes in complex eukaryotes, we have undertaken an extensive mutational analysis in the one cell stage Caenorhabditis elegans embryo. The large size and optical properties of this cell permit observation of cell division processes with great detail in live specimens by simple differential interference contrast (DIC) microscopy. We have screened an extensive collection of maternal-effect embryonic lethal mutations on chromosome III with time-lapse DIC video microscopy. Using this assay, we have identified 48 mutations in 34 loci which are required for specific cell division processes in the one cell stage embryo. We show that mutations fall into distinct phenotypic classes which correspond, among others, to the processes of pronuclear migration, rotation of centrosomes and associated pronuclei, spindle assembly, chromosome segregation, anaphase spindle positioning, and cytokinesis. We have further analyzed pronuclear migration mutants by indirect immunofluorescence microscopy using antibodies against tubulin and ZYG-9, a centrosomal marker. This analysis revealed that two pronuclear migration loci are required for generating normal microtubule arrays and four for centrosome separation. All 34 loci have been mapped by deficiencies to distinct regions of chromosome III, thus paving the way for their rapid molecular characterization. Our work contributes to establishing the one cell stage C. elegans embryo as a powerful metazoan model system for dissecting cell division processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.