ArticleThe Biochemical journal1999
Transcriptional regulation of the human asparagine synthetase gene by carbohydrate availability.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Characterizing asparagine synthetase deficiency variants in lymphoblastoid cell lines.JIMD reports · 2023Article
- Human APOE ɛ3 and APOE ɛ4 Alleles Have Differential Effects on Mouse Olfactory Epithelium.Journal of Alzheimer's disease : JAD · 2022Article
- Enhanced expression of asparagine synthetase under glucose-deprived conditions promotes esophageal squamous cell carcinoma development.International journal of medical sciences · 2020Article
- Glutamate and α-ketoglutarate: key players in glioma metabolism.Amino acids · 2017Review
- Loss of asparagine synthetase suppresses the growth of human lung cancer cells by arresting cell cycle at G0/G1 phase.Cancer gene therapy · 2016Article
- Asparagine synthetase: regulation by cell stress and involvement in tumor biology.American journal of physiology. Endocrinology and metabolism · 2013Review
- Despite increased ATF4 binding at the C/EBP-ATF composite site following activation of the unfolded protein response, system A transporter 2 (SNAT2) transcription activity is repressed in HepG2 cells.The Journal of biological chemistry · 2008Article
- Alignment of the transcription start site coincides with increased transcriptional activity from the human asparagine synthetase gene following amino acid deprivation of HepG2 cells.The Journal of nutrition · 2006Article
- Asparagine synthetase chemotherapy.Annual review of biochemistry · 2006Review
- Nutritional control of gene expression: how mammalian cells respond to amino acid limitation.Annual review of nutrition · 2005Review
- Induction of p21 and p27 expression by amino acid deprivation of HepG2 human hepatoma cells involves mRNA stabilization.The Biochemical journal · 2004Article
- Article
- Characterization of the nutrient-sensing response unit in the human asparagine synthetase promoter.The Biochemical journal · 2003Article
- The immunosuppressant rapamycin mimics a starvation-like signal distinct from amino acid and glucose deprivation.Molecular and cellular biology · 2002Article
- Glucose limitation induces GCN4 translation by activation of Gcn2 protein kinase.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Transcription of the asparagine synthetase (AS) gene is induced by amino acid deprivation. The present data illustrate that this gene is also under transcriptional control by carbohydrate availability. Incubation of human HepG2 hepatoma cells in glucose-free medium resulted in an increased AS mRNA content, reaching a maximum of about 14-fold over control cells after approx. 12 h. Extracellular glucose caused the repression of the content of AS mRNA in a concentration-dependent manner, with a k1/2 (concentration causing a half-maximal repression) of 1 mM. Fructose, galactose, mannose, 2-deoxyglucose and xylitol were found to maintain the mRNA content of both AS and the glucose-regulated protein GRP78 in a state of repression, whereas 3-O-methylglucose did not. Incubation in either histidine-free or glucose-free medium also resulted in adaptive regulation of the AS gene in BNL-CL.2 mouse hepatocytes, rat C6 glioma cells and human MOLT4 lymphocytes, in addition to HepG2 cells. In contrast, the steady-state mRNA content of GRP78 was unaffected by amino acid availability. Transient transfection assays using a reporter gene construct documented that glucose deprivation increases AS gene transcription via elements within the proximal 3 kbp of the AS promoter. These results illustrate that human AS gene transcription is induced following glucose limitation of the cells.
Indexed as
Identifiers
10085239PMC1220139W2068631909What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.