ArticleThe Biochemical journal1999
cDNA cloning and expression of rat homeobox gene, Hex, and functional characterization of the protein.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- An HHEX/IKKα positive feedback loop promotes intestinal inflammation.The Journal of clinical investigation · 2026Article
- The Haematopoietically-expressed homeobox transcription factor: roles in development, physiology and disease.Frontiers in immunology · 2023Review
- Article
- Utility of Common Marmoset (Genes · 2020Review
- Homeobox protein Hhex negatively regulates Treg cells by inhibiting Foxp3 expression and function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Effect of smoking on the association of HHEX (rs5015480) with diabetes among Korean women and heavy smoking men.BMC medical genetics · 2018Article
- Hhex Is Necessary for the Hepatic Differentiation of Mouse ES Cells and Acts via Vegf Signaling.PloS one · 2016Article
- The tumor suppressor HHEX inhibits axon growth when prematurely expressed in developing central nervous system neurons.Molecular and cellular neurosciences · 2015Article
- Hhex is Required at Multiple Stages of Adult Hematopoietic Stem and Progenitor Cell Differentiation.Stem cells (Dayton, Ohio) · 2015Article
- Regulation of liver growth by glypican 3, CD81, hedgehog, and Hhex.The American journal of pathology · 2013Article
- A potential role for the homeoprotein Hhex in hepatocellular carcinoma progression.Medical oncology (Northwood, London, England) · 2012Article
- Study of intermolecular contacts in the proline-rich homeodomain (PRH)-DNA complex using molecular dynamics simulations.European biophysics journal : EBJ · 2012Article
- Interaction between Hhex and SOX13 modulates Wnt/TCF activity.The Journal of biological chemistry · 2010Article
- CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding.Nucleic acids research · 2009Article
- The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors.The Biochemical journal · 2009Article
- The emerging genetic architecture of type 2 diabetes.Cell metabolism · 2008Review
- PRH/Hex: an oligomeric transcription factor and multifunctional regulator of cell fate.The Biochemical journal · 2008Review
- The homeobox gene Hhex is essential for proper hepatoblast differentiation and bile duct morphogenesis.Developmental biology · 2007Article
- Cloning and characterization of mr-s, a novel SAM domain protein, predominantly expressed in retinal photoreceptor cells.BMC developmental biology · 2006Article
- The proline-rich homeodomain (PRH/HEX) protein is down-regulated in liver during infection with lymphocytic choriomeningitis virus.Journal of virology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated two cDNA clones of rat Hex, a homeobox protein, studied its expression in rat liver and various cells, and characterized the protein. The levels of Hex mRNA were only slightly increased in liver of rats refed with a high-carbohydrate diet or after partial hepatectomy. Whereas the expression of Hex mRNA was detected in hepatocytes isolated from adult rat liver and also in highly differentiated hepatoma cells, no Hex mRNA was detected in poorly differentiated hepatoma cells. Hex mRNA was also detected in liver from embryo aged 15 days. Expression of Hex was increased in F9 cells during differentiation into visceral endoderm cells by treatment with retinoic acid. This stimulation occurred prior to an increase in the level of alpha-fetoprotein mRNA. When fusion-protein expression vectors of GAL4 DNA-binding domain and Hex were co-transfected with luciferase reporter plasmid, with or without five copies of the GAL4-binding site, into HepG2 cells, the luciferase activities were decreased in concentration- and GAL4-binding site-dependent manners. This repression did not require the presence of the homeodomain, which is located between the amino acid residues 137 and 196. Its repression domain was mapped between the residues 45 and 136 in the proline-rich N-terminal region. In addition, the homeodomain was responsible for DNA-binding of Hex. These results indicate that Hex functions as a transcriptional repressor and may be involved in the differentiation and/or maintenance of the differentiated state in hepatocytes.
Indexed as
Identifiers
10085234PMC1220134W1984644685What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.