ArticleMolecular and cellular biology1999
Maturation of the myogenic program is induced by postmitotic expression of insulin-like growth factor I.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 153 citations in OpenAlex.
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- Multiple ways to promote adult grass carp (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses.International journal of molecular sciences · 2025Article
- Impaired myoblast differentiation and muscle IGF-1 receptor signaling pathway activation after N-glycosylation inhibition.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Brown to White Fat Transition Overlap With Skeletal Muscle During Development of Larger Mammals: Is it a Coincidence?Journal of the Endocrine Society · 2022Review
- Effects of the ProbioticLife (Basel, Switzerland) · 2022Article
- Effect of Pinoresinol and Vanillic Acid Isolated fromMolecules (Basel, Switzerland) · 2022Article
- Modified UCN2 peptide treatment improves skeletal muscle mass and function in mouse models of obesity-induced insulin resistance.Journal of cachexia, sarcopenia and muscle · 2021Article
- Taurine Attenuates Catabolic Processes Related to the Onset of Sarcopenia.International journal of molecular sciences · 2020Article
- Review
- Tissue-Engineered Human Myobundle System as a Platform for Evaluation of Skeletal Muscle Injury Biomarkers.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Inhibition of Phosphoinositide 3-Kinase/Protein Kinase B Signaling Hampers the Vasopressin-dependent Stimulation of Myogenic Differentiation.International journal of molecular sciences · 2019Article
- Effects of IGF-1 isoforms on muscle growth and sarcopenia.Aging cell · 2019Article
- An Overview about the Biology of Skeletal Muscle Satellite Cells.Current genomics · 2019Review
- Molecular Insights into Muscle Homeostasis, Atrophy and Wasting.Current genomics · 2018Review
- Stem Cells and Tissue Niche: Two Faces of the Same Coin of Muscle Regeneration.European journal of translational myology · 2016Article
- Action of obestatin in skeletal muscle repair: stem cell expansion, muscle growth, and microenvironment remodeling.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Expression profile of IGF-I-calcineurin-NFATc3-dependent pathway genes in skeletal muscle during early development between duck breeds differing in growth rates.Development genes and evolution · 2015Article
- MicroRNAs modulated by local mIGF-1 expression in mdx dystrophic mice.Frontiers in aging neuroscience · 2015Article
- Postmitotic Expression of SOD1(G93A) Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation.Mediators of inflammation · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanisms underlying myogenic induction by insulin-like growth factor I (IGF-I) are distinct from its proliferative effects on myoblasts. To determine the postmitotic role of IGF-I on muscle cell differentiation, we derived L6E9 muscle cell lines carrying a stably transfected rat IGF-I gene under the control of a myosin light chain (MLC) promoter-enhancer cassette. Expression of MLC-IGF-I exclusively in differentiated L6E9 myotubes, which express the embryonic form of myosin heavy chain (MyHC) and no endogenous IGF-I, resulted in pronounced myotube hypertrophy, accompanied by activation of the neonatal MyHC isoform. The hypertrophic myotubes dramatically increased expression of myogenin, muscle creatine kinase, beta-enolase, and IGF binding protein 5 and activated the myocyte enhancer factor 2C gene which is normally silent in this cell line. MLC-IGF-I induction in differentiated L6E9 cells also increased the expression of a transiently transfected LacZ reporter driven by the myogenin promoter, demonstrating activation of the differentiation program at the transcriptional level. Nuclear reorganization, accumulation of skeletal actin protein, and an increased expression of beta1D integrin were also observed. Inhibition of the phosphatidyl inositol (PI) 3-kinase intermediate in IGF-I-mediated signal transduction confirmed that the PI 3-kinase pathway is required only at early stages for IGF-I-mediated hypertrophy and neonatal MyHC induction in these cells. Expression of IGF-I in postmitotic muscle may therefore play an important role in the maturation of the myogenic program.
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Registered trials
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