ArticleMolecular and cellular biology1999
An activator binding module of yeast RNA polymerase II holoenzyme.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 161 citations in OpenAlex.
- Article
- Med3-mediated NADPH generation to helpApplied and environmental microbiology · 2024Article
- Yeast Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism.Molecular cell · 2022Article
- Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II.PLoS genetics · 2020Article
- Twenty years of Mediator complex structural studies.Biochemical Society transactions · 2019Review
- The Structures of Eukaryotic Transcription Pre-initiation Complexes and Their Functional Implications.Sub-cellular biochemistry · 2019Review
- Article
- Transcriptome Analysis of FourG3 (Bethesda, Md.) · 2018Article
- Targeted Recruitment of the Basal Transcriptional Machinery by LNK Clock Components Controls the Circadian Rhythms of Nascent RNAs in Arabidopsis.The Plant cell · 2018Article
- Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex.Biochemistry research international · 2018Article
- Mediator Tail Module Is Required for Tac1-ActivatedAntimicrobial agents and chemotherapy · 2017Article
- Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.Transcription · 2017Review
- Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo.Molecular cell · 2016Article
- Amplification of TLO Mediator Subunit Genes Facilitate Filamentous Growth in Candida Spp.PLoS genetics · 2016Article
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- A functional portrait of Med7 and the mediator complex in Candida albicans.PLoS genetics · 2014Article
- ERK phosphorylation of MED14 in promoter complexes during mitogen-induced gene activation by Elk-1.Nucleic acids research · 2013Article
- Mechanisms of Mediator complex action in transcriptional activation.Cellular and molecular life sciences : CMLS · 2013Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Mediator complex of Saccharomyces cerevisiae is required for both general and regulated transcription of RNA polymerase II (PolII) and is composed of two stable subcomplexes (Srb4 and Rgr1 subcomplexes). To decipher the function of each Mediator subcomplex and to delineate the functional relationship between the subcomplexes, we characterized the compositions and biochemical activities of PolII-Mediator complexes (holoenzymes) prepared from several Mediator mutant strains of S. cerevisiae. We found that holoenzymes devoid of a functional Gal11 module were defective for activated but not basal transcription in a reconstituted in vitro system. This activation-specific defect was correlated with a crippled physical interaction to transcriptional activator proteins, which could be bypassed by artificial recruitment of a mutant holoenzyme to a promoter. Consistent with this observation, a direct interaction between Gal11 and gene-specific transcriptional activator proteins was detected by far-Western analyses and column binding assays. In contrast, the srb5 deletion mutant holoenzyme was defective for both basal and activated transcription, despite its capacity for activator binding that is comparable to that of the wild-type holoenzyme. These results demonstrate that the Gal11 module of the Rgr1 subcomplex is required for the efficient recruitment of PolII holoenzyme to a promoter via activator-specific interactions, while the Srb4 subcomplex functions in the modulation of general polymerase activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.