ArticleMolecular and cellular biology1999
MOT1 can activate basal transcription in vitro by regulating the distribution of TATA binding protein between promoter and nonpromoter sites.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.PLoS genetics · 2021Article
- Conformational changes and catalytic inefficiency associated with Mot1-mediated TBP-DNA dissociation.Nucleic acids research · 2019Article
- The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription through Preinitiation Complex Assembly and Nucleosome Organization.The Journal of biological chemistry · 2016Article
- High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation.Nature structural & molecular biology · 2013Article
- Interconversion between active and inactive TATA-binding protein transcription complexes in the mouse genome.Nucleic acids research · 2012Article
- Genome-wide transcriptional dependence on conserved regions of Mot1.Molecular and cellular biology · 2011Article
- Article
- Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium.Functional & integrative genomics · 2010Article
- Proteomics reveals a physical and functional link between hepatocyte nuclear factor 4alpha and transcription factor IID.The Journal of biological chemistry · 2009Article
- How eukaryotic genes are transcribed.Critical reviews in biochemistry and molecular biology · 2009Review
- TATA-binding protein variants that bypass the requirement for Mot1 in vivo.The Journal of biological chemistry · 2009Article
- A proteomics analysis of yeast Mot1p protein-protein associations: insights into mechanism.Molecular & cellular proteomics : MCP · 2008Article
- Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome.Genes & development · 2008Article
- Computational modelling of genome-wide [corrected] transcription assembly networks using a fluidics analogy.PloS one · 2008Article
- Regulation of rRNA synthesis by TATA-binding protein-associated factor Mot1.Molecular and cellular biology · 2007Article
- Snf1-dependent and Snf1-independent pathways of constitutive ADH2 expression in Saccharomyces cerevisiae.Genetics · 2006Article
- Helicase89B is a Mot1p/BTAF1 homologue that mediates an antimicrobial response in Drosophila.EMBO reports · 2005Article
- NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.Molecular and cellular biology · 2004Article
- Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.Microbiology and molecular biology reviews : MMBR · 2004Review
- High-affinity DNA binding by a Mot1p-TBP complex: implications for TAF-independent transcription.The EMBO journal · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
MOT1 is an ATPase which can dissociate TATA binding protein (TBP)-DNA complexes in a reaction requiring ATP hydrolysis. Consistent with this observation, MOT1 can repress basal transcription in vitro. Paradoxically, however, some genes, such as HIS4, appear to require MOT1 as an activator of transcription in vivo. To further investigate the function of MOT1 in basal transcription, we performed in vitro transcription reactions using yeast nuclear extracts depleted of MOT1. Quantitation of MOT1 revealed that it is an abundant protein, with nuclear extracts from wild-type cells containing a molar excess of MOT1 over TBP. Surprisingly, MOT1 can weakly activate basal transcription in vitro. This activation by MOT1 is detectable with amounts of MOT1 that are approximately stoichiometric to TBP. With amounts of MOT1 similar to those present in wild-type nuclear extracts, MOT1 behaves as a weak repressor of basal transcription. These results suggest that MOT1 might activate transcription via an indirect mechanism in which limiting TBP can be liberated from nonpromoter sites for use at promoters. In support of this idea, excess nonpromoter DNA sequesters TBP and represses transcription, but this effect can be reversed by addition of MOT1. These results help to reconcile previous in vitro and in vivo results and expand the repertoire of transcriptional control strategies to include factor-assisted redistribution of TBP between promoter and nonpromoter sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.