ArticleMolecular and cellular biology1999
An in vitro system recapitulates chromatin remodeling at the PHO5 promoter.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Structural Features of Transcription Factors Associating with Nucleosome Binding.Molecular cell · 2019Article
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
- In vitro reconstitution of PHO5 promoter chromatin remodeling points to a role for activator-nucleosome competition in vivo.Molecular and cellular biology · 2010Article
- Combining chemical genetics and proteomics to identify protein kinase substrates.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains.Comparative and functional genomics · 2005Article
- Affinity purification of specific chromatin segments from chromosomal loci in yeast.Molecular and cellular biology · 2003Article
- Phosphate transport and sensing in Saccharomyces cerevisiae.Genetics · 2001Article
- Remodeling of yeast CUP1 chromatin involves activator-dependent repositioning of nucleosomes over the entire gene and flanking sequences.Molecular and cellular biology · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae gene PHO5 is an excellent system with which to study regulated changes in chromatin structure. The PHO5 promoter is packaged into four positioned nucleosomes under repressing conditions; upon induction, the structure of these nucleosomes is altered such that the promoter DNA becomes accessible to nucleases. We report here the development and characterization of an in vitro system in which partially purified PHO5 minichromosomes undergo promoter chromatin remodeling. Several hallmarks of the PHO5 chromatin transition in vivo were reproduced in this system. Chromatin remodeling of PHO5 minichromosomes required the transcription factors Pho4 and Pho2, was localized to the promoter region of PHO5, and was independent of the chromatin-remodeling complex Swi-Snf. In vitro chromatin remodeling also required the addition of fractionated nuclear extract and hydrolyzable ATP. This in vitro system should serve as a useful tool for identifying the components required for this reaction and for elucidating the mechanism by which the PHO5 promoter chromatin structure is changed.
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