ArticleMolecular and cellular biology1999
Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Cryo-EM analyses of dimerized spliceosomes provide new insights into the functions of B complex proteins.The EMBO journal · 2024Article
- Structural dynamics of the N-terminal domain and the Switch loop of Prp8 during spliceosome assembly and activation.Nucleic acids research · 2018Review
- Article
- Genome-wide profiling of mouse RNA secondary structures reveals key features of the mammalian transcriptome.Genome biology · 2014Article
- Post-transcriptional modifications modulate conformational dynamics in human U2-U6 snRNA complex.RNA (New York, N.Y.) · 2014Article
- RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.The EMBO journal · 2013Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Functionally important structural elements of U12 snRNA.Nucleic acids research · 2011Article
- Single-molecule analysis of protein-free U2-U6 snRNAs.Nature structural & molecular biology · 2009Article
- Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.Molecular bioSystems · 2009Review
- "Nought may endure but mutability": spliceosome dynamics and the regulation of splicing.Molecular cell · 2008Review
- Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.RNA (New York, N.Y.) · 2007Article
- Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts.RNA (New York, N.Y.) · 2007Article
- Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.RNA (New York, N.Y.) · 2007Article
- Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.RNA (New York, N.Y.) · 2006Article
- Prp8 protein: at the heart of the spliceosome.RNA (New York, N.Y.) · 2005Review
- Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA.Molecular and cellular biology · 2005Article
- Multiple functions for the invariant AGC triad of U6 snRNA.RNA (New York, N.Y.) · 2004Article
- Comparative analysis detects dependencies among the 5' splice-site positions.RNA (New York, N.Y.) · 2004Article
- Mutations in U5 snRNA loop 1 influence the splicing of different genes in vivo.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The function of conserved regions of the metazoan U5 snRNA was investigated by reconstituting U5 small nuclear ribonucleoprotein particles (snRNPs) from purified snRNP proteins and HeLa or Xenopus U5 snRNA mutants and testing their ability to restore splicing to U5-depleted nuclear extracts. Substitution of conserved nucleotides comprising internal loop 2 or deletion of internal loop 1 had no significant effect on the ability of reconstituted U5 snRNPs to complement splicing. However, deletion of internal loop 2 abolished U5 activity in splicing and spliceosome formation. Surprisingly, substitution of the invariant loop 1 nucleotides with a GAGA tetraloop had no effect on U5 activity. Furthermore, U5 snRNPs reconstituted from an RNA formed by annealing the 5' and 3' halves of the U5 snRNA, which lacked all loop 1 nucleotides, complemented both steps of splicing. Thus, in contrast to yeast, loop 1 of the human U5 snRNA is dispensable for both steps of splicing in HeLa nuclear extracts. This suggests that its function can be compensated for in vitro by other spliceosomal components: for example, by proteins associated with the U5 snRNP. Consistent with this idea, immunoprecipitation studies indicated that several functionally important U5 proteins associate stably with U5 snRNPs containing a GAGA loop 1 substitution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.