ArticleMolecular and cellular biology1999
Regulation of V(D)J recombination by transcriptional promoters.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- DNA double-strand breaks relieve USF-mediated repression of Dβ2 germline transcription in developing thymocytes.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- The center of accessibility: Dβ control of V(D)J recombination.Archivum immunologiae et therapiae experimentalis · 2010Review
- Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- TCR beta feedback signals inhibit the coupling of recombinationally accessible V beta 14 segments with DJ beta complexes.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Promoter activity 5' of Dbeta2 is coordinated by E47, Runx1, and GATA-3.Molecular immunology · 2009Article
- Article
- Functional overlap in the cis-acting regulation of the V(D)J recombination at the TCRbeta locus.Molecular immunology · 2009Article
- Activation of 12/23-RSS-dependent RAG cleavage by hSWI/SNF complex in the absence of transcription.Molecular cell · 2008Article
- Genomic organization and recombinational unit duplication-driven evolution of ovine and bovine T cell receptor gamma loci.BMC genomics · 2008Article
- Productive coupling of accessible Vbeta14 segments and DJbeta complexes determines the frequency of Vbeta14 rearrangement.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Elucidation of IgH intronic enhancer functions via germ-line deletion.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Patterns of gene divergence and VL promoter activity in immunoglobulin light chain clusters of the channel catfish.Immunogenetics · 2004Article
- B lineage-specific regulation of V(D)J recombinase activity is established in common lymphoid progenitors.The Journal of experimental medicine · 2004Article
- The T cell receptor beta enhancer promotes access and pairing of Dbeta and Jbeta gene segments during V(D)J recombination.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- T cell receptor (TCR) alpha/delta locus enhancer identity and position are critical for the assembly of TCR delta and alpha variable region genes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Cancer predisposition and hematopoietic failure in Rad50(S/S) mice.Genes & development · 2002Article
- The kappa B transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study.BMC immunology · 2002Article
- V(D)J recombination becomes accessible.The Journal of experimental medicine · 2001Article
- Regulation of antigen receptor gene assembly in lymphocytes.Immunologic research · 2001Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Enhancer elements potentiate the rearrangement of antigen receptor loci via changes in the accessibility of gene segment clusters to V(D)J recombinase. Here, we show that enhancer activity per se is insufficient to target T-cell receptor beta miniloci for DbetaJbeta recombination. Instead, a promoter situated 5' to Dbeta1 (PDbeta) was required for efficient rearrangement of chromosomal substrates. A critical function for promoters in regulating gene segment accessibility was further supported by the ability of heterologous promoters to direct rearrangement of enhancer-containing substrates. Importantly, activation of a synthetic tetracycline-inducible promoter (Ptet) positioned upstream from the Dbeta gene segment was sufficient to target recombination of miniloci lacking a distal enhancer element. The latter result suggests that DNA loops, generated by interactions between flanking promoter and enhancer elements, are not required for efficient recognition of chromosomal gene segments by V(D)J recombinase. Unexpectedly, the Ptet substrate exhibited normal levels of rearrangement despite its retention of a hypermethylated DNA status within the DbetaJbeta cluster. Together, our findings support a model in which promoter activation, rather than intrinsic properties of enhancers, is the primary determinant for regulating recombinational accessibility within antigen receptor loci.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.