ArticleMolecular and cellular biology1999
Reciprocal interaction between two cellular proteins, Puralpha and YB-1, modulates transcriptional activity of JCVCY in glial cells.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
- JC virus small tumor antigen promotes S phase entry and cell cycle progression.Tumour virus research · 2024Article
- Article
- The Molecular Function of PURA and Its Implications in Neurological Diseases.Frontiers in genetics · 2021Review
- Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo.Protoplasma · 2017Article
- Review
- Electrostatic and Hydrophobic Interactions Mediate Single-Stranded DNA Recognition and Acta2 Repression by Purine-Rich Element-Binding Protein B.Biochemistry · 2016Article
- New insights on human polyomavirus JC and pathogenesis of progressive multifocal leukoencephalopathy.Clinical & developmental immunology · 2013Review
- Molecular biology, epidemiology, and pathogenesis of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain.Clinical microbiology reviews · 2012Review
- JC virus variant associated with cerebellar atrophy in a patient with AIDS.Journal of clinical microbiology · 2011Article
- JC virus-induced Progressive Multifocal Leukoencephalopathy.Future virology · 2010Article
- JC virus: an oncogenic virus in animals and humans?Seminars in cancer biology · 2009Review
- Mechanism of strand-specific smooth muscle alpha-actin enhancer interaction by purine-rich element binding protein B (Purbeta).Biochemistry · 2009Article
- Multiple roles for Puralpha in cellular and viral regulation.Cell cycle (Georgetown, Tex.) · 2009Review
- Protective role of Puralpha to cisplatin.Cancer biology & therapy · 2008Article
- YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules.BMC biochemistry · 2008Article
- C9orf10 protein, a novel protein component of Puralpha-containing mRNA-protein particles (Puralpha-mRNPs): characterization of developmental and regional expressions in the mouse brain.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Dephosphorylation of JC virus agnoprotein by protein phosphatase 2A: inhibition by small t antigen.Virology · 2008Article
- Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription.The Journal of biological chemistry · 2008Article
- Significance of the Y-box proteins in human cancers.Journal of molecular and genetic medicine : an international journal of biomedical research · 2005Article
- Puralpha is essential for postnatal brain development and developmentally coupled cellular proliferation as revealed by genetic inactivation in the mouse.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cross communication between regulatory proteins is an important event in the control of eukaryotic gene transcription. Here we have examined the structural and functional interaction between two cellular regulatory proteins, YB-1 and Puralpha, on the 23-bp sequence element derived from the enhancer-promoter of the human polyomavirus JCV. YB-1 and Puralpha are single-stranded DNA binding proteins which recognize C/T- and GC/GA-rich sequences, respectively. Results from band shift studies demonstrated that while both proteins interact directly with their DNA target sequences within the 23-bp motif, each protein can regulate the association of the other one with the DNA. Affinity chromatography and coimmunoprecipitation provide evidence for a direct interaction between Puralpha and YB-1 in the absence of the DNA sequence. Ectopic expression of YB-1 and Puralpha in glial cells synergistically stimulated viral promoter activity via the 23-bp sequence element. Results from mutational studies revealed that residues between amino acids 75 and 203 of YB-1 and between amino acids 85 and 215 of Puralpha are important for the interaction between these two proteins. Functional studies with glial cells indicated that the region within Puralpha which mediates its association with YB-1 and binding to the 23-bp sequence is important for the observed activation of the JCV promoter by the Puralpha and YB-1 proteins. The results of this study suggest that the cooperative interaction between YB-1 and Puralpha mediates the synergistic activation of the human polyomavirus JCV genome by these cellular proteins. The importance of these findings for cellular and viral genes which are regulated by Puralpha and YB-1 is discussed.
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