ArticleMolecular and cellular biology1999
Distinct factors regulate the murine RAG-2 promoter in B- and T-cell lines.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 70 citations in OpenAlex.
- Hierarchical assembly and disassembly of a transcriptionally active RAG locus in CD4The Journal of experimental medicine · 2019Article
- Corrected and Republished from: BCL11A Is a Critical Component of a Transcriptional Network That Activates RAG Expression and V(D)J Recombination.Molecular and cellular biology · 2018Article
- The Complex Interplay between DNA Injury and Repair in Enzymatically Induced Mutagenesis and DNA Damage in B Lymphocytes.International journal of molecular sciences · 2017Review
- The BCL11A transcription factor directly activates RAG gene expression and V(D)J recombination.Molecular and cellular biology · 2013Article
- Reciprocal regulation of Rag expression in thymocytes by the zinc-finger proteins, Zfp608 and Zfp609.Genes and immunity · 2013Article
- Article
- Transcription factors E2A, FOXO1 and FOXP1 regulate recombination activating gene expression in cancer cells.PloS one · 2011Article
- Allelic exclusion of immunoglobulin genes: models and mechanisms.Immunological reviews · 2010Review
- Immunoglobulin expression in non-lymphoid lineage and neoplastic cells.The American journal of pathology · 2009Review
- Regulation of B cell fate commitment and immunoglobulin heavy-chain gene rearrangements by Ikaros.Nature immunology · 2008Article
- High-mobility-group A1 (HMGA1) proteins down-regulate the expression of the recombination activating gene 2 (RAG2).The Biochemical journal · 2005Article
- A role for nuclear factor kappa B/rel transcription factors in the regulation of the recombinase activator genes.Immunity · 2005Article
- The role of recombination activating gene (RAG) reinduction in thymocyte development in vivo.The Journal of experimental medicine · 2001Article
- c-Myb binds to a sequence in the proximal region of the RAG-2 promoter and is essential for promoter activity in T-lineage cells.Molecular and cellular biology · 2000Article
- BSAP can repress enhancer activity by targeting PU.1 function.Molecular and cellular biology · 2000Article
- RAG2 is regulated differentially in B and T cells by elements 5' of the promoter.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The recombination activating genes RAG-1 and RAG-2 are expressed in a lymphoid-cell-specific and developmentally regulated fashion. To understand the transcriptional basis for this regulation, we have cloned and characterized the murine RAG-2 promoter. The promoter was lymphoid cell specific, showing activity in various B- and T-cell lines but little activity in nonlymphoid cells. To our surprise, however, the promoter was regulated differently in B and T cells. Using nuclear extracts from B-cell lines, we found that the B-cell-specific transcription factor BSAP (Pax-5) could bind to a conserved sequence critical for promoter activity. BSAP activated the promoter in transfected cells, and the BSAP site was occupied in a tissue-specific manner in vivo. An overlapping DNA sequence binding to a distinct factor was necessary for promoter activity in T cells. Full promoter activity in T cells was also dependent on a more distal DNA sequence whose disruption had no effect on B-cell activity. The unexpected finding that a B-cell-specific factor regulates the RAG-2 promoter may explain some of the recently observed differences in the regulation of RAG transcription between B and T cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.