ArticleMolecular and cellular biology1999
Ku-dependent nonhomologous DNA end joining in Xenopus egg extracts.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 80 citations in OpenAlex.
- Core NHEJ Factors Maintain Synapsis Across Diverse DNA End Structures.bioRxiv : the preprint server for biology · 2026Article
- DNA polymerase λ autoinhibition is relieved via Ku interaction during non-homologous end joining.Nucleic acids research · 2026Article
- Structural role for DNA Ligase IV in promoting the fidelity of non-homologous end joining.Nature communications · 2024Article
- Review
- Repair of DNA Double-Strand Breaks by the Nonhomologous End Joining Pathway.Annual review of biochemistry · 2021Review
- Polθ promotes the repair of 5'-DNA-protein crosslinks by microhomology-mediated end-joining.Cell reports · 2021Article
- Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks.Frontiers in cell and developmental biology · 2021Review
- A Mechanism to Minimize Errors during Non-homologous End Joining.Molecular cell · 2020Article
- A single XLF dimer bridges DNA ends during nonhomologous end joining.Nature structural & molecular biology · 2018Article
- The Human T-Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor Attenuates Repair of Double-Stranded DNA Breaks via Nonhomologous End Joining.Journal of virology · 2018Article
- The capacity of oocytes for DNA repair.Cellular and molecular life sciences : CMLS · 2018Review
- Cip29 is phosphorylated following activation of the DNA damage response in Xenopus egg extracts.PloS one · 2017Article
- Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts.Methods in enzymology · 2017Article
- The structure of ends determines the pathway choice and Mre11 nuclease dependency of DNA double-strand break repair.Nucleic acids research · 2016Article
- Non-homologous end joining repair in Xenopus egg extract.Scientific reports · 2016Article
- Two-Stage Synapsis of DNA Ends during Non-homologous End Joining.Molecular cell · 2016Article
- Mechanistic analysis of Xenopus EXO1's function in 5'-strand resection at DNA double-strand breaks.Nucleic acids research · 2011Article
- Replication protein A promotes 5'-->3' end processing during homology-dependent DNA double-strand break repair.The Journal of cell biology · 2011Article
- Origin-dependent initiation of DNA replication within telomeric sequences.Nucleic acids research · 2010Article
- The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis.Nucleic acids research · 2010Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An extract from activated Xenopus eggs joins both matching and nonmatching ends of exogenous linear DNA substrates with high efficiency and fidelity (P. Pfeiffer and W. Vielmetter, Nucleic Acids Res. 16:907-924, 1988). In mammalian cells, such nonhomologous end joining (NHEJ) is known to require the Ku heterodimer, a component of DNA-dependent protein kinase. Here I investigated whether Ku is also required for the in vitro reaction in the egg extract. Immunological assays indicate that Ku is very abundant in the extract. I found that all NHEJ was inhibited by autoantibodies against Ku and that NHEJ between certain combinations of DNA ends was also decreased after immunodepletion of Ku from the extract. The formation of a joint between a DNA end with a 5'-protruding single strand (PSS) and an end with a 3'-PSS, between two ends with 3'-PSS, and between two blunt ends was most Ku dependent. On the other hand, NHEJ between two DNA ends bearing 5'-PSS was Ku independent. These results show that the Xenopus cell-free system will be useful to biochemically dissect the role of Ku in eukaryotic NHEJ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.