ArticleMolecular and cellular biology1999
Myogenic basic helix-loop-helix proteins and Sp1 interact as components of a multiprotein transcriptional complex required for activity of the human cardiac alpha-actin promoter.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 82 citations in OpenAlex.
- Next-generation direct reprogramming.Frontiers in cell and developmental biology · 2024Review
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- Characterising open chromatin in chick embryos identifies cis-regulatory elements important for paraxial mesoderm formation and axis extension.Nature communications · 2021Article
- The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- Genome-Wide Identification and Expression Profiles of Myosin Genes in the Pacific White Shrimp,Frontiers in physiology · 2019Article
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- Discovery and validation of information theory-based transcription factor and cofactor binding site motifs.Nucleic acids research · 2017Article
- MicroRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1.Molecular and cellular biochemistry · 2016Article
- Extracellular deposition of matrilin-2 controls the timing of the myogenic program during muscle regeneration.Journal of cell science · 2014Article
- Auto-regulation of the Sohlh1 gene by the SOHLH2/SOHLH1/SP1 complex: implications for early spermatogenesis and oogenesis.PloS one · 2014Article
- Comparative expression profiling identifies differential roles for Myogenin and p38α MAPK signaling in myogenesis.Journal of molecular cell biology · 2012Article
- CORECLUST: identification of the conserved CRM grammar together with prediction of gene regulation.Nucleic acids research · 2012Article
- GATA4 regulates ANF expression synergistically with Sp1 in a cardiac hypertrophy model.Journal of cellular and molecular medicine · 2011Article
- SMYD1, the myogenic activator, is a direct target of serum response factor and myogenin.Nucleic acids research · 2009Article
- Sp1 and Sp3 regulate transcription of the cyclin-dependent kinase 5 regulatory subunit 2 (p39) promoter in neuronal cells.Biochimica et biophysica acta · 2009Article
- Myocyte-specific M-CAT and MEF-1 elements regulate G-protein gamma 3 gene (gamma3) expression in cardiac myocytes.DNA and cell biology · 2008Article
- The myocardin-related transcription factor, MASTR, cooperates with MyoD to activate skeletal muscle gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Predicting combinatorial binding of transcription factors to regulatory elements in the human genome by association rule mining.BMC bioinformatics · 2007Article
- The basic helix-loop-helix transcription factor NeuroD1 facilitates interaction of Sp1 with the secretin gene enhancer.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of the human cardiac alpha-actin (HCA) promoter in skeletal muscle cells requires the integrity of DNA binding sites for the serum response factor (SRF), Sp1, and the myogenic basic helix-loop-helix (bHLH) family. In this study we report that activation of the HCA correlates with formation of a muscle-specific multiprotein complex on the promoter. We provide evidence that proteins eluted from the multiprotein complex specifically react with antibodies directed against myogenin, Sp1, and SRF and that the complex can be assembled in vitro by using the HCA promoter and purified MyoD, E12, SRF, and Sp1. In vitro and in vivo assays revealed a direct association of Sp1 and myogenin-MyoD mediated by the DNA-binding domain of Sp1 and the HLH motif of myogenin. The results obtained in this study indicate that protein-protein interactions and the cooperative DNA binding of transcriptional activators are critical steps in the formation of a transcriptionally productive multiprotein complex on the HCA promoter and suggest that the same mechanisms might be utilized to regulate the transcription of muscle-specific and other genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.