ArticleMolecular and cellular biology1999
Requirement of RBP9, a Drosophila Hu homolog, for regulation of cystocyte differentiation and oocyte determination during oogenesis.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 59 citations in OpenAlex.
- A transcriptomics-based RNAi screen for regulators of meiosis and early stages of oocyte development in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
- Two distinct waves of transcriptome and translatome changes drive Drosophila germline stem cell differentiation.The EMBO journal · 2024Article
- The molecular genetics of nELAVL in brain development and disease.European journal of human genetics : EJHG · 2023Review
- Sex Lethal Gene Manipulates Gonadal Development of Medaka,International journal of molecular sciences · 2022Article
- The long noncoding RNAScience advances · 2022Article
- Regulation of the Alternative Neural Transcriptome by ELAV/Hu RNA Binding Proteins.Frontiers in genetics · 2022Review
- Alterations of RNA-binding proteinFrontiers in molecular neuroscience · 2022Article
- Overlapping Activities of ELAV/Hu Family RNA Binding Proteins Specify the Extended Neuronal 3' UTR Landscape in Drosophila.Molecular cell · 2020Article
- Tis11 mediated mRNA decay promotes the reacquisition of Drosophila intestinal stem cell quiescence.Developmental biology · 2017Article
- Concentration and Localization of Coexpressed ELAV/Hu Proteins Control Specificity of mRNA Processing.Molecular and cellular biology · 2015Article
- Model-based analysis for qualitative data: an application in Drosophila germline stem cell regulation.PLoS computational biology · 2014Article
- Barrier mechanisms in the Drosophila blood-brain barrier.Frontiers in neuroscience · 2014Review
- The insulator protein Suppressor of Hairy-wing is an essential transcriptional repressor in the Drosophila ovary.Development (Cambridge, England) · 2013Article
- Optimising homing endonuclease gene drive performance in a semi-refractory species: the Drosophila melanogaster experience.PloS one · 2013Article
- Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling.Development (Cambridge, England) · 2012Article
- Brat promotes stem cell differentiation via control of a bistable switch that restricts BMP signaling.Developmental cell · 2011Article
- HuB (elavl2) mRNA is restricted to the germ cells by post-transcriptional mechanisms including stabilisation of the message by DAZL.PloS one · 2011Article
- Drosophila ataxin 2-binding protein 1 marks an intermediate step in the molecular differentiation of female germline cysts.Development (Cambridge, England) · 2010Article
- A functional genomic screen in planarians identifies novel regulators of germ cell development.Genes & development · 2010Article
- A glutamine-rich factor affects stem cell genesis in leech.Stem cells international · 2010Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila RNA binding protein RBP9 and its Drosophila and human homologs, ELAV and the Hu family of proteins, respectively, are highly expressed in the nuclei of neuronal cells. However, biochemical studies suggest that the Hu proteins function in the regulation of mRNA stability, which occurs in the cytoplasm. In this paper, we show that RBP9 is expressed not only in the nuclei of neuronal cells but also in the cytoplasm of cystocytes during oogenesis. Despite the predominant expression of RBP9 in nerve cells, mutational analysis revealed a female sterility phenotype rather than neuronal defects for Rbp9 mutants. The female sterility phenotype of the Rbp9 mutants resulted from defects in oogenesis; the lack of Rbp9 activity caused the germarium region of the mutants to be filled with undifferentiated cystocytes. RBP9 appears to stimulate cystocyte differentiation by regulating the expression of bag-of-marbles (bam) mRNA, which encodes a developmental regulator of germ cells. RBP9 protein bound specifically to bam mRNA in vitro, which is required for cystocyte proliferation, and the number of cells that expressed BAM protein was increased 5- to 10-fold in the germarium regions of Rbp9 mutants. These results suggest that RBP9 protein binds to bam mRNA to down regulate BAM protein expression, which is essential for the initiation of cystocyte differentiation into functional egg chambers. In hypomorphic Rbp9 mutants, cystocytes differentiated into egg chambers; however, oocyte determination and positioning were perturbed. Therefore, the concentrated localization of RBP9 protein in the oocyte of the early egg chambers may be required for proper oocyte determination or positioning.
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