ArticlePlant molecular biology1999
Isolation and characterization of an anther-specific gene, RA8, from rice (Oryza sativa L.).
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Genome-Wide Analysis of BURP Domain-Containing Gene Family inInternational journal of molecular sciences · 2024Article
- VOZ1 and VOZ2 transcription factors regulate arsenic tolerance and distribution in rice and Arabidopsis.Frontiers in plant science · 2023Article
- Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response.BMC genomics · 2022Article
- Amplification of LTRs of extrachromosomal linear DNAs (ALE-seq) identifies two active Oryco LTR retrotransposons in the rice cultivar Dongjin.Mobile DNA · 2022Article
- Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton.International journal of molecular sciences · 2022Article
- Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: current state and future prospects.Planta · 2021Review
- A Rapid Pipeline for Pollen- and Anther-Specific Gene Discovery Based on Transcriptome Profiling Analysis of Maize Tissues.International journal of molecular sciences · 2021Article
- Identification of the Core Pollen-Specific Regulation in the RiceInternational journal of molecular sciences · 2020Article
- Chromosome Pairing in Polyploid Grasses.Frontiers in plant science · 2020Review
- Genome-wide identification and expression analysis of the BURP domain-containing genes in Gossypium hirsutum.BMC genomics · 2019Article
- A quantitative proteomic analysis of the molecular mechanism underlying fertility conversion in thermo-sensitive genetic male sterility line AnnongS-1.BMC plant biology · 2019Article
- OsMAPKKK63 is involved in salt stress response and seed dormancy control.Plant signaling & behavior · 2019Article
- Chromatin interacting factor OsVIL2 increases biomass and rice grain yield.Plant biotechnology journal · 2019Article
- Engineering rice with lower grain arsenic.Plant biotechnology journal · 2018Article
- The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves.Rice (New York, N.Y.) · 2018Article
- Article
- Genome-Wide Transcription During Early Wheat Meiosis Is Independent of Synapsis, Ploidy Level, and theFrontiers in plant science · 2018Article
- Rice Phytochrome-Interacting Factor-Like1 (OsPIL1) is involved in the promotion of chlorophyll biosynthesis through feed-forward regulatory loops.Journal of experimental botany · 2017Article
- Exploiting theMolecular breeding : new strategies in plant improvement · 2017Article
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6 authors.
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Abstract
An anther-specific cDNA clone of rice, RA8, was isolated from an anther cDNA library by differential screening. RNA blot analysis indicated that the RA8 transcript is present specifically in anthers and the transcript level increased as flowers matured, reaching the highest level in mature flowers. The RA8 clone contains an open reading frame of 264 amino acid residues with a hydrophobic N-terminal region. The deduced amino acid sequences did not show significant homology to any known sequences. Genomic DNA blot analysis showed that RA8 is a single-copy gene. A genomic clone corresponding to the RA8 cDNA was isolated and its promoter region was fused to the beta-glucuronidase (GUS) gene. Transgenic rice plants exhibited anther-specific expression of the GUS reporter gene. Histochemical GUS analysis showed that the RA8 promoter was active in the tapetum, endothecium, and connective tissues of anthers. Experiments showed that expression of the gene starts when microspores are released from tetrads, and it reaches to the maximum level at the late vacuolated-pollen stage. The RA8 promoter may be useful for controlling gene expression in anthers of cereal plants and for generating male-sterile plants.
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