Evidence map›Paper›PMID 10080704›Full record

ArticlePlant molecular biology1999

Cloning and characterization of the nuclear AC115 gene of Chlamydomonas reinhardtii.

P Rattanachaikunsopon, C Rosch, M R Kuchka

Abstract read
PubMed Publisher
In one paragraph

Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Assembly and Repair of Photosystem II inPlants (Basel, Switzerland) · 2024
    Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

P RattanachaikunsoponDepartment of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA.
C Rosch
M R Kuchka

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nuclear ac115 mutant of Chlamydomonas reinhardtii is specifically blocked in the synthesis of the chloroplast encoded D2 protein of the photosystem II reaction center at a point after translation initiation. Here, we report the identification of the AC115 gene through complementation rescue of the ac115 mutant strain, using an indexed cosmid library of Chlamydomonas genomic DNA. AC115 is a small, novel, intronless nuclear gene which encodes a protein of 113 amino acids. The amino terminal end of the Ac115 protein is rich in basic amino acids and has features which resemble a chloroplast transit sequence. A hydrophobic stretch of amino acids at the protein's carboxyl terminus is sufficiently large to be a membrane spanning or a protein/protein interaction domain. Various models are discussed to account for the mechanism by which Ac115p works in D2 synthesis. The ac115 mutant allele was sequenced and determined to be an A-to-T transversion at the first position of the fourth codon of the coding sequence. This mutation changes an AAG codon to a TAG nonsense codon and results in a null phenotype.

Indexed as

Algal ProteinsAmino Acid SequenceAnimalsBase SequenceBiological TransportBlotting, SouthernChlamydomonas reinhardtiiCloning, MolecularCosmidsDeoxyribonucleases, Type II Site-SpecificDNA, PlantDNA, ProtozoanMolecular Sequence DataNuclear ProteinsPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexAC115 protein, Chlamydomonas reinhardtiiAlgal ProteinsCTGCAG-specific type II deoxyribonucleasesDeoxyribonucleases, Type II Site-SpecificDNA, PlantDNA, ProtozoanNuclear ProteinsPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexPlant Proteins

Identifiers

What OpenQuestion holds

Texttitle and abstract
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.