Evidence map›Paper›PMID 10080137›Full record

ArticleDigestive diseases and sciences1999

Helicobacter pylori in dental plaque: a comparison of different PCR primer sets.

Q Song, B Haller, R M Schmid, G Adler, G Bode

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Digestive diseases and sciences, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 2 pooled it
4.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 75 citations in OpenAlex.

  1. Recurrent aphthous stomatitis and Helicobacter pylori.Medicina oral, patologia oral y cirugia bucal · 2016
    Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Article
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  7. Article
  8. Current Prevalence of OralPathogens (Basel, Switzerland) · 2020
    Article
  9. Dentists Are at a Higher Risk for OralBioMed research international · 2020
    Article
  10. Article
  11. [Analysis of the infection status of saliva Helicobacter pylori in Lanzhou].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2014
    Article
  12. Review
  13. Detection of Helicobacter pylori in Oral Lesions.Journal of dental research, dental clinics, dental prospects · 2013
    Article
  14. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Q SongDepartment of Internal Medicine I, University of Ulm, Germany.
B Haller
R M Schmid
G Adler
G Bode
Universität Ulm · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study was designed to compare different primer sets for PCR analysis of H. pylori in the same series of 40 dental plaque samples. Three pairs of primers, HPU1/HPU2, HP1/HP2, and EHC-U/EHC-L, directed to the urease A gene, 16S rRNA gene, or 860-bp DNA of H. pylori, respectively, were used. Our results demonstrate that EHC-L/EHC-U were more specific and sensitive for H. pylori added to saliva or dental plaque than HPU1/HPU2 and HP1/HP2. The detection rates for H. pylori DNA in dental plaque samples from randomly selected adult patients from the Dental Clinic of the University of Ulm were 26.5% (9/34) for HPU1/HPU2, 78.9% (30/38) for HP1/HP2, and 100% (40/40) for EHC-U/EHC-L (P < 0.001). Nested PCR using primers directed to the 860-bp DNA of H. pylori further confirmed the presence of H. pylori DNA (40/40) in all these samples. Our results indicate that primers EHC-U/EHC-L are to be recommended for PCR detection of H. pylori in the oral cavity.

Indexed as

AdultDental PlaqueDNA, BacterialDNA PrimersFemaleHelicobacter pyloriHumansMalePolymerase Chain ReactionRandom AllocationSalivaSensitivity and SpecificityDNA, BacterialDNA Primers

Identifiers

PMID10080137
OpenAlexW129600154

What OpenQuestion holds

Texttitle and abstract
measurements read3
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.