ArticleThe American journal of pathology1999
Role of glutaraldehyde in calcification of porcine aortic valve fibroblasts.
Article in The American journal of pathology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 94 citations in OpenAlex.
- Mid-term valve performance and durability of the Ozaki procedure in patients on chronic dialysis.JTCVS open · 2026Article
- Cell Calcification Models and Their Implications for Medicine and Biomaterial Research.Advanced healthcare materials · 2026Review
- Ectopic Calcification in Congenital Heart Surgery: A Material-Centric Review.Pediatric cardiology · 2025Review
- Smaller Bioprosthetic Valves May Be Associated with Worse Clinical Outcomes and Reduced Freedom from Reoperation in sAVR.Journal of cardiovascular development and disease · 2025Review
- Multiscale structure and function of the aortic valve apparatus.Physiological reviews · 2024Review
- Breaking the Limit of Cardiovascular Regenerative Medicine: Successful 6-Month Goat Implant in World's First Ascending Aortic Replacement Using Biotube Blood Vessels.Bioengineering (Basel, Switzerland) · 2024Article
- Article
- Graft rejection in paediatric congenital heart disease.Translational pediatrics · 2023Review
- Soft Biomimetic Approach for the Development of Calcinosis-Resistant Glutaraldehyde-Fixed Biomaterials for Cardiovascular Surgery.Biomimetics (Basel, Switzerland) · 2023Article
- Chemical modification of acellular fish skin as a promising biological scaffold by carbodiimide cross-linker for wound healing.International wound journal · 2023Article
- Bioprosthetic heart valve structural degeneration associated with metabolic syndrome: Mitigation with polyoxazoline modification.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Development and testing of a transcatheter heart valve with reduced calcification potential.Frontiers in cardiovascular medicine · 2023Article
- Early and midterm outcomes of a bentall operation using an all-biological valved BioConduit™.Journal of cardiothoracic surgery · 2022Article
- Adventitial Collagen Cross-Linking by Glutaraldehyde Reinforcing Human Saphenous Vein - Implication for Coronary Artery Bypass Grafting.Brazilian journal of cardiovascular surgery · 2022Article
- Mechanisms and Drug Therapies of Bioprosthetic Heart Valve Calcification.Frontiers in pharmacology · 2022Review
- Surgical techniques for aortic valve xenotransplantation.Journal of cardiothoracic surgery · 2021Article
- In-vivo assessment of a tissue engineered vascular graft computationally optimized for target vessel compliance.Acta biomaterialia · 2021Article
- Decellularized tissue-engineered heart valves calcification: what do animal and clinical studies tell us?Journal of materials science. Materials in medicine · 2020Review
- Degeneration of Bioprosthetic Heart Valves: Update 2020.Journal of the American Heart Association · 2020Review
- Small Diameter Xenogeneic Extracellular Matrix Scaffolds for Vascular Applications.Tissue engineering. Part B, Reviews · 2020Review
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutaraldehyde-treated porcine aortic valve xenografts frequently fail due to calcification. Calcification in the prostheses begins intracellularly. In a previous study, various types of cell injury to canine valvular fibroblasts, including glutaraldehyde treatment, led to calcification. An influx of extracellular Ca2+ into the phosphate-rich cytosol was theorized to be the mechanism of calcification. To test the Ca2+ influx theory, cytosolic Ca2+ and Pi concentrations were assessed in glutaraldehyde-treated porcine aortic valve fibroblasts, and their relationship to a subsequent calcification was studied. Glutaraldehyde caused an immediate and sustained massive cytosolic Ca2+ increase that was dose dependent and a several-fold increase in Pi. Calcification of cells followed within a week. The earliest calcification was observed in blebs formed on glutaraldehyde-treated cells. Live control cells or cells fixed with glutaraldehyde in Ca2+-free solution did not calcify under the same conditions. Concomitant increases in Ca2+ and Pi in glutaraldehyde-treated cells appear to underlie the mechanism of calcification, and the presence of extracellular Ca2+ during glutaraldehyde fixation promotes calcification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.