ArticleProceedings of the National Academy of Sciences of the United States of America1999
The role of an alternative sigma factor in motility and pilus formation in the cyanobacterium Synechocystis sp. strain PCC6803.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 150 citations in OpenAlex.
- Type IV pilin regulation: a transcriptional overview.Critical reviews in microbiology · 2026Review
- A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways.Biofilm · 2024Article
- Control of light-dependent behaviour in cyanobacteria by the second messenger cyclic di-GMP.microLife · 2023Review
- Article
- Cyanobacteria: Model Microorganisms and Beyond.Microorganisms · 2022Review
- Article
- The Role of the Cyanobacterial Type IV Pilus Machinery in Finding and Maintaining a Favourable Environment.Life (Basel, Switzerland) · 2020Review
- Function and Benefits of Natural Competence in Cyanobacteria: From Ecology to Targeted Manipulation.Life (Basel, Switzerland) · 2020Review
- The circadian clock and darkness control natural competence in cyanobacteria.Nature communications · 2020Article
- Information integration and collective motility in phototactic cyanobacteria.PLoS computational biology · 2020Article
- The Stress-Responsive Alternative Sigma Factor SigB ofFrontiers in microbiology · 2020Review
- Recent Advances in Biological Functions of Thick Pili in the CyanobacteriumFrontiers in plant science · 2020Review
- Article
- Cyanobacteria Respond to Low Levels of Ethylene.Frontiers in plant science · 2019Article
- Sycrp2 Is Essential for Twitching Motility in the Cyanobacterium Synechocystis sp. Strain PCC 6803.Journal of bacteriology · 2018Article
- Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1.Journal of bacteriology · 2018Article
- Ethylene causes transcriptomic changes inPlant direct · 2018Article
- The Small Regulatory Antisense RNA PilR Affects Pilus Formation and Cell Motility by Negatively RegulatingFrontiers in microbiology · 2018Article
- Construction of a Shuttle Vector Using an Endogenous Plasmid From the Cyanobacterium Synechocystis sp. PCC6803.Frontiers in microbiology · 2018Article
- Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Disruption of a gene for an alternative sigma factor, designated sigF, in the freshwater, unicellular cyanobacterium Synechocystis sp. strain PCC6803 resulted in a pleiotropic phenotype. Most notably, this mutant lost phototactic movement with a concomitant loss of pili, which are abundant on the surface of wild-type cells. The sigF mutant also secreted both high levels of yellow-brown and UV-absorbing pigments and a polypeptide that is similar to a large family of extracellular proteins that includes the hemolysins. Furthermore, the sigF mutant had a dramatically reduced level of the transcript from two tandemly arranged pilA genes (sll1694 and sll1695), which encode major structural components of type IV pili. Inactivation of these pilA genes eliminated phototactic movement, though some pili were still present in this strain. Together, these results demonstrate that SigF plays a critical role in motility via the control of pili formation and is also likely to regulate other features of the cell surface. Furthermore, the data provide evidence that type IV pili are required for phototactic movement in certain cyanobacteria and suggest that different populations of pili present on the Synechocystis cell surface may perform different functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.