ArticleProceedings of the National Academy of Sciences of the United States of America1999
Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- A microfluidic device for isolating intact chromosomes from single mammalian cells and probing their folding stability by controlling solution conditions.Scientific reports · 2018Article
- DNA methylation in gastric cancer, related to Helicobacter pylori and Epstein-Barr virus.World journal of gastroenterology · 2014Review
- Lineage-specific function of Engrailed-2 in the progression of chronic myelogenous leukemia to T-cell blast crisis.Cell cycle (Georgetown, Tex.) · 2014Article
- Differential epigenetic regulation of TOX subfamily high mobility group box genes in lung and breast cancers.PloS one · 2012Article
- Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing.BMC clinical pathology · 2011Article
- Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation.Nucleic acids research · 2006Article
- Heterogeneity in the modification and involvement of chromatin components of the CpG island of the silenced human CDH1 gene in cancer cells.Nucleic acids research · 2002Article
- A comprehensive catalog of CpG islands methylated in human lung adenocarcinomas for the identification of tumor suppressor genes.Oncogene · 2002Article
- HOX gene clusters are hotspots of de novo methylation in CpG islands of human lung adenocarcinomas.Oncogene · 2002Article
- A genome-wide screen for normally methylated human CpG islands that can identify novel imprinted genes.Genome research · 2002Article
- A novel technique for the identification of CpG islands exhibiting altered methylation patterns (ICEAMP).Nucleic acids research · 2001Article
- Structure of the highly conserved HERC2 gene and of multiple partially duplicated paralogs in human.Genome research · 2000Article
- Inhibition of DNA methyltransferase stimulates the expression of signal transducer and activator of transcription 1, 2, and 3 genes in colon tumor cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Global methylation profiling of lung cancer identifies novel methylated genes.Neoplasia (New York, N.Y.)Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have constructed a library of DNA fragments heavily methylated in human adenocarcinomas of the lung to permit the comprehensive isolation of methylated CpG islands in cancer. Heavily methylated genomic DNA fragments from tumors of nine male patients were enriched using a methylated DNA binding column and used for construction of the library. From this library, DNA fragments having properties of CpG islands were isolated on the basis of their reduced rate of strand dissociation during denaturing gradient gel electrophoresis. Approximately 1,000 clones, corresponding to 0.3% of the library were analyzed, and nine DNA fragments were identified as being associated with CpG islands that were methylated in tumor DNA. One CpG island was methylated specifically in tumor DNA, whereas the remaining eight CpG islands were methylated both in normal and tumor DNA derived from the same patients. Our results suggest that the number of CpG islands methylated specifically in tumors is not large. The library, which contains DNA fragments from methylated CpG islands comprehensively, is expected to be valuable when elucidating epigenetic processes involved in carcinogenesis.
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