ArticleProceedings of the National Academy of Sciences of the United States of America1999
An activation-specific role for transcription factor TFIIB in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.Genes & development · 2016Article
- Müller glia cells activation in rat retina after optic nerve injury: spatiotemporal correlation with transcription initiation factor IIb.Journal of molecular neuroscience : MN · 2013Article
- Involvement of transcription initiation factor IIB in the light-induced death of rat retinal ganglion cells in vivo.Journal of molecular histology · 2013Article
- Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping.The Journal of biological chemistry · 2011Article
- Article
- Improved Tet-responsive promoters with minimized background expression.BMC biotechnology · 2010Article
- An RNA-based transcription activator derived from an inhibitory aptamer.Nucleic acids research · 2010Article
- Phosphorylation of TFIIB links transcription initiation and termination.Current biology : CB · 2010Article
- Mediator subunits and histone methyltransferase Set2 contribute to Ino2-dependent transcriptional activation of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Article
- The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein.The Journal of biological chemistry · 2008Article
- TFIIB and the regulation of transcription by RNA polymerase II.Chromosoma · 2007Review
- A conformational change in TFIIB is required for activator-mediated assembly of the preinitiation complex.Nucleic acids research · 2004Article
- TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism.Nucleic acids research · 2004Article
- Regulation of phosphate acquisition in Saccharomyces cerevisiae.Current genetics · 2003Review
- Core promoter-dependent TFIIB conformation and a role for TFIIB conformation in transcription start site selection.Molecular and cellular biology · 2002Article
- The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells.Nucleic acids research · 2002Article
- Activator-mediated disruption of sequence-specific DNA contacts by the general transcription factor TFIIB.Genes & development · 2001Article
- Promoter-specific shifts in transcription initiation conferred by yeast TFIIB mutations are determined by the sequence in the immediate vicinity of the start sites.Molecular and cellular biology · 2001Article
- The two Saccharomyces cerevisiae SUA7 (TFIIB) transcripts differ at the 3'-end and respond differently to stress.Nucleic acids research · 2000Article
- Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription.Protein science : a publication of the Protein Society · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A yeast mutant was isolated encoding a single amino acid substitution [serine-53 --> proline (S53P)] in transcription factor TFIIB that impairs activation of the PHO5 gene in response to phosphate starvation. This effect is activation-specific because S53P did not affect the uninduced level of PHO5 expression, yet is not specific to PHO5 because Adr1-mediated activation of the ADH2 gene also was impaired by S53P. Pho4, the principal activator of PHO5, directly interacted with TFIIB in vitro, and this interaction was impaired by the S53P replacement. Furthermore, Pho4 induced a conformational change in TFIIB, detected by enhanced sensitivity to V8 protease. The S53P replacement also impaired activation of a lexA(op)-lacZ reporter by a LexA fusion protein to the activation domain of Adr1, thereby indicating that the transcriptional effect on ADH2 expression is specific to the activation function of Adr1. These results define an activation-specific role for TFIIB in vivo and suggest that certain activators induce a conformational change in TFIIB as part of their mechanism of transcriptional stimulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.